Bioaerosol sampling of a ventilated patient with COVID-19

•Recent research has found SARS-CoV-2 in the air of hospital patient rooms and common areas; however, there has not been research in the rooms of patients on ventilators.•Our study sought to determine whether SARS-CoV-2 was present in the patient room of a COVID-19 positive patient on a ventilator.•This study found that the level of SARS-CoV-2 in the air of the patient room was lower than a detectable level.•This research contributes to our understanding of the spread of COVID-19 in hospital settings and has implications for recommendations for PPE use in patient rooms of individuals on ventilators. Bioaerosol samples were collected in an airborne infection isolation room, bathroom, and anteroom of a ventilated patient with coronavirus disease 2019. Twenty-eight samples were negative for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid, possibly due to the patient being on a closed-circuit ventilator or the efficiency of the air exchanges in the room.