lncRNA RP11-838N2.3 Promoted Cisplatin Resistance in Lung Adenocarcinoma

The mechanism of RP11-838N2.3 promoting cisplatin resistance in lung adenocarcinoma (LAD) was unclear. The RP11-838N2.3 expression level in cells and LAD tissues was detected by qPCR. We constructed lentivirus-mediated GV303 overexpression and GV248 shRNA vector targeting RP11-838N2.3, then infected...

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Veröffentlicht in:	BioMed research international 2020, Vol.2020 (2020), p.1-18
Hauptverfasser:	Wang, Yumin, Wang, Junjun, Zhang, Fan, Hu, Lijuan, Jiang, Feng, Chen, Jie, Huang, Kate
Format:	Artikel
Sprache:	eng
Schlagworte:	A549 Cells Adenocarcinoma Adenocarcinoma of Lung - chemistry Adenocarcinoma of Lung - genetics Adenocarcinoma of Lung - metabolism Adenocarcinoma of Lung - mortality Antineoplastic Agents - pharmacology Apoptosis Biopsy Cancer therapies Cell adhesion & migration Cell culture Cell cycle Cell Cycle - drug effects Cell migration Cell Survival - drug effects Cells Chemotherapy Cisplatin Cisplatin - pharmacology DNA microarrays Drug resistance Drug Resistance, Neoplasm - drug effects Drug Resistance, Neoplasm - genetics Female Genes Humans Lung - chemistry Lung cancer Lung Neoplasms - chemistry Lung Neoplasms - genetics Lung Neoplasms - metabolism Lung Neoplasms - mortality Lungs Male Medical prognosis RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism Survival Tissues Transcriptome Transfection
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Zusammenfassung:	The mechanism of RP11-838N2.3 promoting cisplatin resistance in lung adenocarcinoma (LAD) was unclear. The RP11-838N2.3 expression level in cells and LAD tissues was detected by qPCR. We constructed lentivirus-mediated GV303 overexpression and GV248 shRNA vector targeting RP11-838N2.3, then infected A549 and A549/DDP cell and furtherly analyzed cell biology. High-throughput gene chip analysis showed that RP11-838N2.3 was significantly upregulated in A549/DDP (change fold=66.056595). The qPCR results showed that the expression level of RP11-838N2.3 in A549/DDP cell was significantly higher than that in A549 cells (P
ISSN:	2314-6133 2314-6141
DOI:	10.1155/2020/2806042