Different effects of total flavonoids from Arachniodes exilis on human umbilical cord mesenchymal stem cells in vitro
Traditional Chinese medicines are used in promotion of fractured bone healing and bone diseases. Some studies reported total flavonoids from plant can be used as an auxiliary source of exogenous.Use different methods to identify and verify effects of total flavonoids from Arachniodes exilis (TFAE) o...
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| Veröffentlicht in: | Medicine (Baltimore) 2020-06, Vol.99 (25), p.e20628-e20628 |
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| creator | Yu, Wenmin Hu, Wenlong Ke, Xiumei Zhou, Xufeng Yin, Changchang Yin, Ming |
| description | Traditional Chinese medicines are used in promotion of fractured bone healing and bone diseases. Some studies reported total flavonoids from plant can be used as an auxiliary source of exogenous.Use different methods to identify and verify effects of total flavonoids from Arachniodes exilis (TFAE) on human umbilical cord mesenchymal stem cells (HUCMSCs) in vitro.Concentrations of 1 and 5 μg/mL TFAE significantly increased ALPase activity in HUCMSCs compared to the other concentrations at days 3 and 7 (P |
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Some studies reported total flavonoids from plant can be used as an auxiliary source of exogenous.Use different methods to identify and verify effects of total flavonoids from Arachniodes exilis (TFAE) on human umbilical cord mesenchymal stem cells (HUCMSCs) in vitro.Concentrations of 1 and 5 μg/mL TFAE significantly increased ALPase activity in HUCMSCs compared to the other concentrations at days 3 and 7 (P < .05). RT-PCR showed that expression levels of osteogenic genes (Col1a1, OPN, Runx2 and Osx) were remarkably enhanced in HUCMSCs following treatment with different concentrations of TFAE for 9 days compared with 0 μg/mL TFAE group (control). The results showed that concentration < 5 μg/mL of TFAE induced osteogenic differentiation in HUCMSCs Alizarin red staining assays revealed that both TFAE and S1191 was significantly decreased (7.80 ± 0.66) compared with the TFAE group (16.00 ± 0.97) (P < .01). ALPase activity on days 3 and 7 was relatively lower in HUCMSCs grown in media supplemented with both S1191 and TFAE than that of in TFAE group only. The results indicated that osteogenic markers (Col1a1, OPN, Runx2 and Osx) were significantly downregulated in the TFAE + S1191 group in comparison to the control group. The expressions of Col1a and OPN in the TFAE + S1191 group decreased significantly (P < .01) by Western blotting.TFAE promotes the odonto/osteogenic differentiation of human UCMSCs via activation of ER.</description><identifier>ISSN: 0025-7974</identifier><identifier>EISSN: 1536-5964</identifier><identifier>DOI: 10.1097/MD.0000000000020628</identifier><identifier>PMID: 32569193</identifier><language>eng</language><publisher>United States: the Author(s). Published by Wolters Kluwer Health, Inc</publisher><subject>Cell Differentiation - drug effects ; Cells, Cultured ; Flavonoids - pharmacology ; Humans ; In Vitro Techniques ; Medicine, Chinese Traditional ; Mesenchymal Stem Cells - drug effects ; Observational Study ; Osteogenesis - drug effects ; Plant Extracts - pharmacology ; Plants, Medicinal - chemistry ; Umbilical Cord - cytology</subject><ispartof>Medicine (Baltimore), 2020-06, Vol.99 (25), p.e20628-e20628</ispartof><rights>the Author(s). Published by Wolters Kluwer Health, Inc.</rights><rights>Copyright © 2020 the Author(s). Published by Wolters Kluwer Health, Inc. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4501-9c0f37177a6735514082ebd1ce5a8b0e17f8ae0e296169f041a989eb341e18b93</citedby><cites>FETCH-LOGICAL-c4501-9c0f37177a6735514082ebd1ce5a8b0e17f8ae0e296169f041a989eb341e18b93</cites><orcidid>0000-0001-7999-0104 ; 0000-0003-3829-4182</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310876/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310876/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32569193$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Wenmin</creatorcontrib><creatorcontrib>Hu, Wenlong</creatorcontrib><creatorcontrib>Ke, Xiumei</creatorcontrib><creatorcontrib>Zhou, Xufeng</creatorcontrib><creatorcontrib>Yin, Changchang</creatorcontrib><creatorcontrib>Yin, Ming</creatorcontrib><title>Different effects of total flavonoids from Arachniodes exilis on human umbilical cord mesenchymal stem cells in vitro</title><title>Medicine (Baltimore)</title><addtitle>Medicine (Baltimore)</addtitle><description>Traditional Chinese medicines are used in promotion of fractured bone healing and bone diseases. Some studies reported total flavonoids from plant can be used as an auxiliary source of exogenous.Use different methods to identify and verify effects of total flavonoids from Arachniodes exilis (TFAE) on human umbilical cord mesenchymal stem cells (HUCMSCs) in vitro.Concentrations of 1 and 5 μg/mL TFAE significantly increased ALPase activity in HUCMSCs compared to the other concentrations at days 3 and 7 (P < .05). RT-PCR showed that expression levels of osteogenic genes (Col1a1, OPN, Runx2 and Osx) were remarkably enhanced in HUCMSCs following treatment with different concentrations of TFAE for 9 days compared with 0 μg/mL TFAE group (control). The results showed that concentration < 5 μg/mL of TFAE induced osteogenic differentiation in HUCMSCs Alizarin red staining assays revealed that both TFAE and S1191 was significantly decreased (7.80 ± 0.66) compared with the TFAE group (16.00 ± 0.97) (P < .01). ALPase activity on days 3 and 7 was relatively lower in HUCMSCs grown in media supplemented with both S1191 and TFAE than that of in TFAE group only. The results indicated that osteogenic markers (Col1a1, OPN, Runx2 and Osx) were significantly downregulated in the TFAE + S1191 group in comparison to the control group. The expressions of Col1a and OPN in the TFAE + S1191 group decreased significantly (P < .01) by Western blotting.TFAE promotes the odonto/osteogenic differentiation of human UCMSCs via activation of ER.</description><subject>Cell Differentiation - drug effects</subject><subject>Cells, Cultured</subject><subject>Flavonoids - pharmacology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Medicine, Chinese Traditional</subject><subject>Mesenchymal Stem Cells - drug effects</subject><subject>Observational Study</subject><subject>Osteogenesis - drug effects</subject><subject>Plant Extracts - pharmacology</subject><subject>Plants, Medicinal - chemistry</subject><subject>Umbilical Cord - cytology</subject><issn>0025-7974</issn><issn>1536-5964</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdUU1v1TAQtBCIvhZ-ARLykUuKv2LHF6SqDyhSKy7lbDnOmhicuNjJK_33uH2ltPiy1nhm1ruD0BtKjinR6v3F9pj8O4xI1j1DG9py2bRaiudoU9G2UVqJA3RYyg9CKFdMvEQHnLVSU803aN0G7yHDvGCoF7cUnDxe0mIj9tHu0pzCULDPacIn2bpxDmmAguF3iKFyZzyuk53xOvUVcFXlUh7wBAVmN95MFSgLTNhBjAWHGe_CktMr9MLbWOD1fT1C3z59vDw9a86_fv5yenLeONES2mhHPFdUKSsVb1sqSMegH6iD1nY9Aap8Z4EA05JK7YmgVncaei4o0K7X_Ah92Pterf0Eg6tjZhvNVQ6TzTcm2WCevsxhNN_TzihOSadkNXh3b5DTrxXKYqZQbmexM6S1GCaoZKpuXlQq31NdTqVk8A9tKDG3gZmLrfk_sKp6-_iHD5q_CVWC2BOuU1wgl59xvYZsRrBxGe_8WqVZw6ofkVSTpiKC8j9Bx6Ku</recordid><startdate>20200619</startdate><enddate>20200619</enddate><creator>Yu, Wenmin</creator><creator>Hu, Wenlong</creator><creator>Ke, Xiumei</creator><creator>Zhou, Xufeng</creator><creator>Yin, Changchang</creator><creator>Yin, Ming</creator><general>the Author(s). Published by Wolters Kluwer Health, Inc</general><general>Wolters Kluwer Health</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7999-0104</orcidid><orcidid>https://orcid.org/0000-0003-3829-4182</orcidid></search><sort><creationdate>20200619</creationdate><title>Different effects of total flavonoids from Arachniodes exilis on human umbilical cord mesenchymal stem cells in vitro</title><author>Yu, Wenmin ; Hu, Wenlong ; Ke, Xiumei ; Zhou, Xufeng ; Yin, Changchang ; Yin, Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4501-9c0f37177a6735514082ebd1ce5a8b0e17f8ae0e296169f041a989eb341e18b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Cell Differentiation - drug effects</topic><topic>Cells, Cultured</topic><topic>Flavonoids - pharmacology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Medicine, Chinese Traditional</topic><topic>Mesenchymal Stem Cells - drug effects</topic><topic>Observational Study</topic><topic>Osteogenesis - drug effects</topic><topic>Plant Extracts - pharmacology</topic><topic>Plants, Medicinal - chemistry</topic><topic>Umbilical Cord - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Wenmin</creatorcontrib><creatorcontrib>Hu, Wenlong</creatorcontrib><creatorcontrib>Ke, Xiumei</creatorcontrib><creatorcontrib>Zhou, Xufeng</creatorcontrib><creatorcontrib>Yin, Changchang</creatorcontrib><creatorcontrib>Yin, Ming</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Medicine (Baltimore)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Wenmin</au><au>Hu, Wenlong</au><au>Ke, Xiumei</au><au>Zhou, Xufeng</au><au>Yin, Changchang</au><au>Yin, Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Different effects of total flavonoids from Arachniodes exilis on human umbilical cord mesenchymal stem cells in vitro</atitle><jtitle>Medicine (Baltimore)</jtitle><addtitle>Medicine (Baltimore)</addtitle><date>2020-06-19</date><risdate>2020</risdate><volume>99</volume><issue>25</issue><spage>e20628</spage><epage>e20628</epage><pages>e20628-e20628</pages><issn>0025-7974</issn><eissn>1536-5964</eissn><abstract>Traditional Chinese medicines are used in promotion of fractured bone healing and bone diseases. Some studies reported total flavonoids from plant can be used as an auxiliary source of exogenous.Use different methods to identify and verify effects of total flavonoids from Arachniodes exilis (TFAE) on human umbilical cord mesenchymal stem cells (HUCMSCs) in vitro.Concentrations of 1 and 5 μg/mL TFAE significantly increased ALPase activity in HUCMSCs compared to the other concentrations at days 3 and 7 (P < .05). RT-PCR showed that expression levels of osteogenic genes (Col1a1, OPN, Runx2 and Osx) were remarkably enhanced in HUCMSCs following treatment with different concentrations of TFAE for 9 days compared with 0 μg/mL TFAE group (control). The results showed that concentration < 5 μg/mL of TFAE induced osteogenic differentiation in HUCMSCs Alizarin red staining assays revealed that both TFAE and S1191 was significantly decreased (7.80 ± 0.66) compared with the TFAE group (16.00 ± 0.97) (P < .01). ALPase activity on days 3 and 7 was relatively lower in HUCMSCs grown in media supplemented with both S1191 and TFAE than that of in TFAE group only. The results indicated that osteogenic markers (Col1a1, OPN, Runx2 and Osx) were significantly downregulated in the TFAE + S1191 group in comparison to the control group. The expressions of Col1a and OPN in the TFAE + S1191 group decreased significantly (P < .01) by Western blotting.TFAE promotes the odonto/osteogenic differentiation of human UCMSCs via activation of ER.</abstract><cop>United States</cop><pub>the Author(s). Published by Wolters Kluwer Health, Inc</pub><pmid>32569193</pmid><doi>10.1097/MD.0000000000020628</doi><orcidid>https://orcid.org/0000-0001-7999-0104</orcidid><orcidid>https://orcid.org/0000-0003-3829-4182</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Cell Differentiation - drug effects Cells, Cultured Flavonoids - pharmacology Humans In Vitro Techniques Medicine, Chinese Traditional Mesenchymal Stem Cells - drug effects Observational Study Osteogenesis - drug effects Plant Extracts - pharmacology Plants, Medicinal - chemistry Umbilical Cord - cytology |
| title | Different effects of total flavonoids from Arachniodes exilis on human umbilical cord mesenchymal stem cells in vitro |
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