How to perform a nasopharyngeal swab in adults and children in the COVID-19 era

The nasopharyngeal swab is currently the main testing method used to diagnose COVID-19. The principle is to collect respiratory cells infected by the virus and to use the RT-PCR (Reverse Transcription - Polymerase Chain Reaction) technique to detect the RNA of the virus. The false negative rate is h...

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Veröffentlicht in:	European annals of otorhinolaryngology, head and neck diseases head and neck diseases, 2020-09, Vol.137 (4), p.325-327
Hauptverfasser:	Pondaven-Letourmy, S., Alvin, F., Boumghit, Y., Simon, F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Betacoronavirus - genetics Betacoronavirus - isolation & purification Child Clinical Laboratory Techniques - methods Coronavirus Infections - diagnosis Coronavirus Infections - virology COVID-19 COVID-19 Testing Diagnosis Humans Nasopharyngeal swab Nasopharynx - virology Pandemics Pneumonia, Viral - diagnosis Pneumonia, Viral - virology Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - analysis RT-PCR SARS-CoV-2
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Beschreibung
Zusammenfassung:	The nasopharyngeal swab is currently the main testing method used to diagnose COVID-19. The principle is to collect respiratory cells infected by the virus and to use the RT-PCR (Reverse Transcription - Polymerase Chain Reaction) technique to detect the RNA of the virus. The false negative rate is high, about 30%, which can mainly be explained by an incorrect execution of the technique may increase the false negative rate and decrease the test's sensitivity. The aim of this note is to help healthcare providers to perform this test correctly in adults and children.
ISSN:	1879-7296 1879-730X
DOI:	10.1016/j.anorl.2020.06.001