Control of Ligand-Binding Specificity Using Photocleavable Linkers in AFM Force Spectroscopy
In recent decades, atomic force microscopy (AFM), in particular the force spectroscopy mode, has become a method of choice to study biomolecular interactions at the single-molecule level. However, grafting procedures as well as determining binding specificity remain challenging. We report here an in...
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Veröffentlicht in: | Nano letters 2020-05, Vol.20 (5), p.4038-4042 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | In recent decades, atomic force microscopy (AFM), in particular the force spectroscopy mode, has become a method of choice to study biomolecular interactions at the single-molecule level. However, grafting procedures as well as determining binding specificity remain challenging. We report here an innovative approach based on a photocleavable group that enables in situ release of the ligands bound to the AFM tip and thus allows direct assessment of the binding specificity. Applicable to a wide variety of molecules, the strategy presented here provides new opportunities to study specific interactions and deliver single molecules with high spatiotemporal resolution in a wide range of applications, including AFM-based cell biology. |
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ISSN: | 1530-6984 1530-6992 |
DOI: | 10.1021/acs.nanolett.0c01426 |