Ultrabright fluorescent nanoscale labels for the femtomolar detection of analytes with standard bioassays

The detection and quantification of low-abundance molecular biomarkers in biological samples is challenging. Here, we show that a plasmonic nanoscale construct serving as an ‘add-on’ label for a broad range of bioassays improves their signal-to-noise ratio and dynamic range without altering their wo...

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Veröffentlicht in:Nature biomedical engineering 2020-05, Vol.4 (5), p.518-530
Hauptverfasser: Luan, Jingyi, Seth, Anushree, Gupta, Rohit, Wang, Zheyu, Rathi, Priya, Cao, Sisi, Gholami Derami, Hamed, Tang, Rui, Xu, Baogang, Achilefu, Samuel, Morrissey, Jeremiah J., Singamaneni, Srikanth
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Sprache:eng
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Zusammenfassung:The detection and quantification of low-abundance molecular biomarkers in biological samples is challenging. Here, we show that a plasmonic nanoscale construct serving as an ‘add-on’ label for a broad range of bioassays improves their signal-to-noise ratio and dynamic range without altering their workflow and readout devices. The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element. Its emission wavelength can be tuned over the visible and near-infrared spectral regions by modifying its size, shape and composition. It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples. A plasmonic nanoscale construct serving as a customizable ‘add-on’ fluorescent label improves the signal-to-noise ratio and dynamic range of a range of fluorescence-based bioassays.
ISSN:2157-846X
2157-846X
DOI:10.1038/s41551-020-0547-4