Antioxidant molecular mechanism of adenosyl homocysteinase from cyanobacteria and its wound healing process in fibroblast cells

An antioxidant molecule namely, adenosyl homocysteinase (AHc) was identified from the earlier constructed transcriptome database of Spirulina, where it was cultured in a sulphur deprived condition. From the AHc protein, a small peptide NL13 was identified using bioinformatics tools and was predicted...

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Veröffentlicht in:	Molecular biology reports 2020-03, Vol.47 (3), p.1821-1834
Hauptverfasser:	Sarkar, Purabi, Stefi, Raju V., Pasupuleti, Mukesh, Paray, Bilal Ahmad, Al-Sadoon, Mohammad K., Arockiaraj, Jesu
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosylhomocysteinase - chemistry Animal Anatomy Animal Biochemistry Animals Antioxidants Antioxidants - chemical synthesis Antioxidants - chemistry Antioxidants - pharmacology Bacterial Proteins - chemistry Bioinformatics Biomedical and Life Sciences Cell adhesion & migration Cell migration Cell Movement - drug effects Cell Proliferation - drug effects Cell Survival Cell viability Chlorocebus aethiops Cytotoxicity Fibroblasts Fibroblasts - cytology Fibroblasts - drug effects Fibroblasts - metabolism Gene expression Histology Humans Intracellular Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - metabolism Life Sciences Morphology Original Original Article Oxidative stress Peptides Peptides - chemical synthesis Peptides - chemistry Peptides - pharmacology Reactive Oxygen Species - metabolism Spirulina - enzymology Sulfur Vero Cells Vitamin E Wound healing Wound Healing - drug effects
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Zusammenfassung:	An antioxidant molecule namely, adenosyl homocysteinase (AHc) was identified from the earlier constructed transcriptome database of Spirulina, where it was cultured in a sulphur deprived condition. From the AHc protein, a small peptide NL13 was identified using bioinformatics tools and was predicted to have antioxidant property. Further, the peptide was synthesised and its antioxidant mechanism was addressed at molecular level. NL13 was subjected to various antioxidant assays including DPPH assay, HARS assay, SARS Assay, NO assay and ABTS assay, where NL13 exhibited significant (P
ISSN:	0301-4851 1573-4978
DOI:	10.1007/s11033-020-05276-y