A stereological study of developmental changes in hepatocyte ultrastructure of zebrafish (Danio rerio)

Histopathology can reveal toxicant‐induced changes in the structure of a tissue or organ. A prerequisite for histopathological studies is a sound knowledge of the morphology of the anatomical structure in the normal or healthy state. Zebrafish larvae can provide a tool for studies focused on hepatotoxicity at early stages of development; therefore, the fine structure of the organ should be well characterised. To date, liver structure at 72 and 120 hr post‐fertilisation (hpf) has not been reported in detail and this study aimed to fill this scientific gap. A stereological approach allowed for quantitative description of the liver and revealed ultrastructural alterations occurring with time of development. These included a significant increase in the absolute volume of hepatocytes, mitochondria and rough endoplasmic reticulum (rER) during the period of study. The surface area of rER, and of outer and inner mitochondrial membranes also increased. There was no change in the absolute volume of the nuclei. This study provides a quantitative spatial and temporal framework for future research aiming to detect early developmental changes in the liver. Zebrafish larvae can provide a tool for studies focused on hepatotoxicity at early stages of development. In the present study hepatocyte ultrastructure was quantified using a stereological approach at 72 and 120 hr post‐fertilization. This study provides a quantitative spatial and temporal framework for future research aiming to detect early developmental changes in the liver.