The Effects of Sulglycotide on the Adhesion and the Inflammation of Helicobacter Pylori

( ) is a primary etiologic factor in gastric diseases. Sulglycotide is a glycopeptide derived from pig duodenal mucin. Esterification of its carbohydrate chains with sulfate groups creates a potent gastroprotective agent used to treat various gastric diseases. We investigated the inhibitory effects of sulglycotide on adhesion and inflammation after infection in human gastric adenocarcinoma cells (AGS cells). reference strain 60190 (ATCC 49503) was cultured on Brucella agar supplemented with 10% bovine serum. Sulgylcotide-mediated growth inhibition of was evaluated using the broth dilution method. Inhibition of adhesion to AGS cells by sulglycotide was assessed using a urease assay. Effects of sulglycotide on the translocation of virulence factors was measured using western blot to detect cytotoxin-associated protein A (CagA) and vacuolating cytotoxin A (VacA) proteins. Inhibition of IL-8 secretion was measured using enzyme-linked immunosorbent assay (ELISA) to determine the effects of sulglycotide on inflammation. Sulglycotide did not inhibit the growth of , however, after six and 12 hours of infection on AGS cells, adhesion was significantly inhibited by approximately 60% by various concentrations of sulglycotide. Sulglycotide decreased virulence factor (CagA and VacA) translocation to AGS cells and inhibited IL-8 secretion. Sulglycotide inhibited adhesion and inflammation after infection of AGS cells in vitro. These results support the use of sulglycotide to treat infections.