Keth-seq for transcriptome-wide RNA structure mapping

Keth-seq for transcriptome-wide RNA structure mapping

RNA secondary structure is critical to RNA regulation and function. We report a new N 3 -kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N 3 -kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-...

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Veröffentlicht in:Nature chemical biology 2020-05, Vol.16 (5), p.489-492
Hauptverfasser: Weng, Xiaocheng, Gong, Jing, Chen, Yi, Wu, Tong, Wang, Fang, Yang, Shixi, Yuan, Yushu, Luo, Guanzheng, Chen, Kai, Hu, Lulu, Ma, Honghui, Wang, Pingluan, Zhang, Qiangfeng Cliff, Zhou, Xiang, He, Chuan
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Sprache:eng
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631/92
631/92/500
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Aldehydes - chemistry
Animals
Biochemical Engineering
Biochemistry
Biology
Bioorganic Chemistry
Brief Communication
Butanones
Cell Biology
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Embryonic Stem Cells
Gene expression
Guanine
Guanine - chemistry
Guanosine
HeLa Cells
High-Throughput Nucleotide Sequencing
Humans
Labeling
Laboratories
Life sciences
Mapping
Mice
Nucleic Acid Conformation
Nucleic Acid Heteroduplexes
Protein structure
Reagents
Ribonucleic acid
RNA
RNA - chemistry
RNA Folding
Secondary structure
Stem cells
Transcriptome
Transcriptomes
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Beschreibung
Zusammenfassung:RNA secondary structure is critical to RNA regulation and function. We report a new N 3 -kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N 3 -kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-wide RNA secondary structure mapping. The authors designed a chemical probe, azido-kethoxal, to specifically label guanosine in single-strand RNAs in live cells that could be used to determine transcriptome-wide RNA secondary structures.
ISSN:1552-4450
1552-4469
DOI:10.1038/s41589-019-0459-3