A rapid, quantitative assay for titration of bovine virus diarrhoea-mucosal disease virus

An end point dilution microtitration assay is described that can be used for the titration of both cytopathic and non-cytopathic isolates of bovine virus diarrhoea-mucosal disease virus. Indirect immunofluorescence is used to detect infected MDBK cells in the wells of Terasaki plates. The virus titr...

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Veröffentlicht in:	Veterinary microbiology 1988-12, Vol.18 (3), p.209-217
Hauptverfasser:	Roberts, Peter C., Etchison, James R., Bond, Clifford W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences BOVIN Bovine Virus Diarrhea-Mucosal Disease - diagnosis Bovine Virus Diarrhea-Mucosal Disease - microbiology CATTLE Cattle Diseases - microbiology DIARREA Diarrhea Viruses, Bovine Viral - growth & development Diarrhea Viruses, Bovine Viral - isolation & purification DIARRHEE DIARRHOEA Fundamental and applied biological sciences. Psychology GANADO BOVINO IDENTIFICACION IDENTIFICATION Microbiology PESTIVIRUS Pestivirus - growth & development Staining and Labeling Techniques used in virology Viral Plaque Assay Virology
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Zusammenfassung:	An end point dilution microtitration assay is described that can be used for the titration of both cytopathic and non-cytopathic isolates of bovine virus diarrhoea-mucosal disease virus. Indirect immunofluorescence is used to detect infected MDBK cells in the wells of Terasaki plates. The virus titre is derived from the number of uninfected wells, using the Poisson distribution. The assay is simple, fast and economical. Titres of cytopathic virus determined by the microtitration assay and standard plaque assay are equivalent.
ISSN:	0378-1135 1873-2542
DOI:	10.1016/0378-1135(88)90088-0