Novel full-length major histocompatibility complex class I allele discovery and haplotype definition in pig-tailed macaques
Pig-tailed macaques ( Macaca nemestrina , Mane ) are important models for human immunodeficiency virus (HIV) studies. Their infectability with minimally modified HIV makes them a uniquely valuable animal model to mimic human infection with HIV and progression to acquired immunodeficiency syndrome (A...
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Veröffentlicht in: | Immunogenetics (New York) 2018-06, Vol.70 (6), p.381-399 |
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Sprache: | eng |
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Zusammenfassung: | Pig-tailed macaques (
Macaca nemestrina
,
Mane
) are important models for human immunodeficiency virus (HIV) studies. Their infectability with minimally modified HIV makes them a uniquely valuable animal model to mimic human infection with HIV and progression to acquired immunodeficiency syndrome (AIDS). However, variation in the pig-tailed macaque major histocompatibility complex (MHC) and the impact of individual transcripts on the pathogenesis of HIV and other infectious diseases is understudied compared to that of rhesus and cynomolgus macaques. In this study, we used Pacific Biosciences single-molecule real-time circular consensus sequencing to describe full-length MHC class I (MHC-I) transcripts for 194 pig-tailed macaques from three breeding centers. We then used the full-length sequences to infer
Mane-A
and
Mane-B
haplotypes containing groups of MHC-I transcripts that co-segregate due to physical linkage. In total, we characterized full-length open reading frames (ORFs) for 313
Mane-A
,
Mane-B
, and
Mane-I
sequences that defined 86
Mane-A
and 106
Mane-B
MHC-I haplotypes. Pacific Biosciences technology allows us to resolve these
Mane-A
and
Mane-B
haplotypes to the level of synonymous allelic variants. The newly defined haplotypes and transcript sequences containing full-length ORFs provide an important resource for infectious disease researchers as certain MHC haplotypes have been shown to provide exceptional control of simian immunodeficiency virus (SIV) replication and prevention of AIDS-like disease in nonhuman primates. The increased allelic resolution provided by Pacific Biosciences sequencing also benefits transplant research by allowing researchers to more specifically match haplotypes between donors and recipients to the level of nonsynonymous allelic variation, thus reducing the risk of graft-versus-host disease. |
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ISSN: | 0093-7711 1432-1211 |
DOI: | 10.1007/s00251-017-1042-2 |