Responses of Auditory Cortex to Complex Stimuli: Functional Organization Revealed Using Intrinsic Optical Signals

1 Department of Neurobiology and the Interdisciplinary Center for Neural Computation, The Hebrew University, Jerusalem, Israel; and 2 Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom Submitted 25 April 2007; accepted in final form 11 February 2008 We used optical imaging of intrinsic signals to study the large-scale organization of ferret auditory cortex in response to complex sounds. Cortical responses were collected during continuous stimulation by sequences of sounds with varying frequency, period, or interaural level differences. We used a set of stimuli that differ in spectral structure, but have the same periodicity and therefore evoke the same pitch percept (click trains, sinusoidally amplitude modulated tones, and iterated ripple noise). These stimuli failed to reveal a consistent periodotopic map across the auditory fields imaged. Rather, gradients of period sensitivity differed for the different types of periodic stimuli. Binaural interactions were studied both with single contralateral, ipsilateral, and diotic broadband noise bursts and with sequences of broadband noise bursts with varying level presented contralaterally, ipsilaterally, or in opposite phase to both ears. Contralateral responses were generally largest and ipsilateral responses were smallest when using single noise bursts, but the extent of the activated area was large and comparable in all three aural configurations. Modulating the amplitude in counter phase to the two ears generally produced weaker modulation of the optical signals than the modulation produced by the monaural stimuli. These results suggest that binaural interactions seen in cortex are most likely predominantly due to subcortical processing. Thus our optical imaging data do not support the theory that the primary or nonprimary cortical fields imaged are topographically organized to form consistent maps of systematically varying sensitivity either to stimulus pitch or to simple binaural properties of the acoustic stimuli. Address for reprint requests and other correspondence: I. Nelken, Dept. of Neurobiology, Institute of Life Sciences, Safra Campus, Givat Ram, Jerusalem 91104, Israel (E-mail: israel{at}cc.huji.ac.il )