Recombinant influenza H1, H5 and H9 hemagglutinins containing replaced H3 hemagglutinin transmembrane domain showed enhanced heterosubtypic protection in mice

Recombinant influenza H1, H5 and H9 hemagglutinins containing replaced H3 hemagglutinin transmembrane domain showed enhanced heterosubtypic protection in mice

Highlights • We found H3-WT transmembrane domain is critical for H3 HA-induced hetero-protection. • Wild-type H3 showed more hetero-protection than H1, H5 and H9 HAs. • Replaced transmembrane domain had no apparent impact on in vitro expression of H1, H5 and H9 HA proteins in Sf9 cells. • HAs with H...

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Veröffentlicht in:Vaccine 2014-05, Vol.32 (25), p.3041-3049
Hauptverfasser: Liu, Qiliang, Liu, Kang, Xue, Chunyi, Zhou, Jianqiang, Li, Xiaoming, Luo, Dongyu, Zheng, Jing, Xu, Shun, Liu, George Dacai, Cao, Yongchang
Format: Artikel
Sprache:eng
Schlagworte:
Allergy and Immunology
Amino Acid Sequence
Animals
Applied microbiology
Avian flu
Binding sites
Biological and medical sciences
Cloning
Cross Protection
Cytokines - immunology
Eggs
Female
Flow cytometry
Fundamental and applied biological sciences. Psychology
Hemagglutination Inhibition Tests
Hemagglutinin Glycoproteins, Influenza Virus - classification
Hemagglutinin Glycoproteins, Influenza Virus - immunology
Immunization
Infections
Influenza A Virus, H1N1 Subtype
Influenza A Virus, H9N2 Subtype
Influenza Vaccines - immunology
Mice
Mice, Inbred BALB C
Microbiology
Miscellaneous
Molecular Sequence Data
Mutagenesis
Mutation
Orthomyxoviridae Infections - prevention & control
Pandemics
Protein Structure, Tertiary
Proteins
Recombinant Proteins - immunology
Sf9 Cells
Survival
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Virology
Viruses
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Zusammenfassung:Highlights • We found H3-WT transmembrane domain is critical for H3 HA-induced hetero-protection. • Wild-type H3 showed more hetero-protection than H1, H5 and H9 HAs. • Replaced transmembrane domain had no apparent impact on in vitro expression of H1, H5 and H9 HA proteins in Sf9 cells. • HAs with H3 transmembrane domain proteins exhibited enhanced heterosubtypic protections.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2014.03.058