Neutralizing antibody and protective immunity to SARS coronavirus infection of mice induced by a soluble recombinant polypeptide containing an N-terminal segment of the spike glycoprotein

A secreted, glycosylated polypeptide containing amino acids 14 to 762 of the SARS coronavirus (SARS-CoV) spike protein and a polyhistidine tag was expressed in recombinant baculovirus-infected insect cells. Mice received the affinity-purified protein with either a saponin (QS21) or a Ribi (MPL + TDM...

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Veröffentlicht in:	Virology (New York, N.Y.) N.Y.), 2005-04, Vol.334 (2), p.160-165
Hauptverfasser:	Bisht, Himani, Roberts, Anjeanette, Vogel, Leatrice, Subbarao, Kanta, Moss, Bernard
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral - blood Baculoviridae Cells, Cultured Glycoprotein HeLa Cells Humans Immunization Membrane Glycoproteins - administration & dosage Membrane Glycoproteins - chemistry Membrane Glycoproteins - genetics Membrane Glycoproteins - immunology Mice Neutralization Tests Peptides - administration & dosage Peptides - chemistry Peptides - genetics Peptides - immunology Polypeptide Recombinant Proteins - administration & dosage Recombinant Proteins - genetics Recombinant Proteins - immunology Respiratory tract SARS coronavirus SARS Virus - immunology SARS Virus - pathogenicity Severe Acute Respiratory Syndrome - immunology Severe Acute Respiratory Syndrome - prevention & control Solubility Spike Glycoprotein, Coronavirus Spodoptera Viral Envelope Proteins - administration & dosage Viral Envelope Proteins - chemistry Viral Envelope Proteins - genetics Viral Envelope Proteins - immunology Viral Vaccines - administration & dosage Viral Vaccines - genetics Viral Vaccines - immunology
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Beschreibung
Zusammenfassung:	A secreted, glycosylated polypeptide containing amino acids 14 to 762 of the SARS coronavirus (SARS-CoV) spike protein and a polyhistidine tag was expressed in recombinant baculovirus-infected insect cells. Mice received the affinity-purified protein with either a saponin (QS21) or a Ribi (MPL + TDM) adjuvant subcutaneously and were challenged intranasally with SARS-CoV. Both regimens induced binding and neutralizing antibodies and protection against SARS-CoV intranasal infection. However, the best results were obtained with QS21 and protein, which provided the highest antibody as well as complete protection of the upper and lower respiratory tract.
ISSN:	0042-6822 1096-0341
DOI:	10.1016/j.virol.2005.01.042