Production of CCHF virus-like particle by a baculovirus-insect cell expression system

Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV w...

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Veröffentlicht in:	Virologica Sinica 2011-10, Vol.26 (5), p.338-346, Article 338
Hauptverfasser:	Zhou, Zhao-rui, Wang, Man-li, Deng, Fei, Li, Tian-xian, Hu, Zhi-hong, Wang, Hua-lin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Baculoviridae Baculoviridae - genetics Baculovirus Biochemistry Biomedical and Life Sciences Biomedicine Blotting, Western Bunyaviridae Cell Line Centrifugation, Density Gradient - methods Crimean hemorrhagic fever Crimean-Congo hemorrhagic fever virus Cytology electron microscopes Electron microscopy Gene Expression Genetic Vectors Hemorrhagic Fever Virus, Crimean-Congo - genetics Insect cells insects Medical Microbiology Microbial Genetics and Genomics Microbiology microscopy Microscopy, Electron, Transmission N protein Nairovirus nucleocapsid Nucleocapsid Proteins - genetics Nucleocapsid Proteins - metabolism Nucleocapsids Oncology proteins Recombinant Proteins - genetics Recombinant Proteins - metabolism Spodoptera Sucrose Ultracentrifugation Virology Virosomes - genetics Virosomes - isolation & purification Virosomes - metabolism Virosomes - ultrastructure Virus-like particles viruses Western blotting
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