Production of CCHF virus-like particle by a baculovirus-insect cell expression system

Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV w...

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Veröffentlicht in:Virologica Sinica 2011-10, Vol.26 (5), p.338-346, Article 338
Hauptverfasser: Zhou, Zhao-rui, Wang, Man-li, Deng, Fei, Li, Tian-xian, Hu, Zhi-hong, Wang, Hua-lin
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Sprache:eng
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Zusammenfassung:Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope, Virus-Like Particles (VLPs) with various size and morphology were observed in cytoplasmic vesicles in the infected cells. Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation, which was confirmed by Western blot analysis and immuno-electron microscopy (IEM).
ISSN:1674-0769
1995-820X
DOI:10.1007/s12250-011-3209-6