Production of CCHF virus-like particle by a baculovirus-insect cell expression system
Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV w...
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| Veröffentlicht in: | Virologica Sinica 2011-10, Vol.26 (5), p.338-346, Article 338 |
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| creator | Zhou, Zhao-rui Wang, Man-li Deng, Fei Li, Tian-xian Hu, Zhi-hong Wang, Hua-lin |
| description | Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope, Virus-Like Particles (VLPs) with various size and morphology were observed in cytoplasmic vesicles in the infected cells. Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation, which was confirmed by Western blot analysis and immuno-electron microscopy (IEM). |
| doi_str_mv | 10.1007/s12250-011-3209-6 |
| format | Article |
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The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope, Virus-Like Particles (VLPs) with various size and morphology were observed in cytoplasmic vesicles in the infected cells. Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation, which was confirmed by Western blot analysis and immuno-electron microscopy (IEM).</description><identifier>ISSN: 1674-0769</identifier><identifier>EISSN: 1995-820X</identifier><identifier>DOI: 10.1007/s12250-011-3209-6</identifier><identifier>PMID: 21979573</identifier><language>eng</language><publisher>Heidelberg: Springer-Verlag</publisher><subject>Animals ; Baculoviridae ; Baculoviridae - genetics ; Baculovirus ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Blotting, Western ; Bunyaviridae ; Cell Line ; Centrifugation, Density Gradient - methods ; Crimean hemorrhagic fever ; Crimean-Congo hemorrhagic fever virus ; Cytology ; electron microscopes ; Electron microscopy ; Gene Expression ; Genetic Vectors ; Hemorrhagic Fever Virus, Crimean-Congo - genetics ; Insect cells ; insects ; Medical Microbiology ; Microbial Genetics and Genomics ; Microbiology ; microscopy ; Microscopy, Electron, Transmission ; N protein ; Nairovirus ; nucleocapsid ; Nucleocapsid Proteins - genetics ; Nucleocapsid Proteins - metabolism ; Nucleocapsids ; Oncology ; proteins ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Spodoptera ; Sucrose ; Ultracentrifugation ; Virology ; Virosomes - genetics ; Virosomes - isolation & purification ; Virosomes - metabolism ; Virosomes - ultrastructure ; Virus-like particles ; viruses ; Western blotting</subject><ispartof>Virologica Sinica, 2011-10, Vol.26 (5), p.338-346, Article 338</ispartof><rights>Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2011</rights><rights>2011© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2011</rights><rights>Copyright © Wanfang Data Co. 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All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4716-457375f682a4379ae424849afc64eeab2457fe0da736d70738df28686fa559403</citedby><cites>FETCH-LOGICAL-c4716-457375f682a4379ae424849afc64eeab2457fe0da736d70738df28686fa559403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/zgbdx/zgbdx.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090442/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090442/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21979573$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Zhao-rui</creatorcontrib><creatorcontrib>Wang, Man-li</creatorcontrib><creatorcontrib>Deng, Fei</creatorcontrib><creatorcontrib>Li, Tian-xian</creatorcontrib><creatorcontrib>Hu, Zhi-hong</creatorcontrib><creatorcontrib>Wang, Hua-lin</creatorcontrib><title>Production of CCHF virus-like particle by a baculovirus-insect cell expression system</title><title>Virologica Sinica</title><addtitle>Virol. Sin</addtitle><addtitle>Virol Sin</addtitle><description>Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope, Virus-Like Particles (VLPs) with various size and morphology were observed in cytoplasmic vesicles in the infected cells. Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation, which was confirmed by Western blot analysis and immuno-electron microscopy (IEM).</description><subject>Animals</subject><subject>Baculoviridae</subject><subject>Baculoviridae - genetics</subject><subject>Baculovirus</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blotting, Western</subject><subject>Bunyaviridae</subject><subject>Cell Line</subject><subject>Centrifugation, Density Gradient - methods</subject><subject>Crimean hemorrhagic fever</subject><subject>Crimean-Congo hemorrhagic fever virus</subject><subject>Cytology</subject><subject>electron microscopes</subject><subject>Electron microscopy</subject><subject>Gene Expression</subject><subject>Genetic Vectors</subject><subject>Hemorrhagic Fever Virus, Crimean-Congo - genetics</subject><subject>Insect cells</subject><subject>insects</subject><subject>Medical Microbiology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>microscopy</subject><subject>Microscopy, Electron, Transmission</subject><subject>N protein</subject><subject>Nairovirus</subject><subject>nucleocapsid</subject><subject>Nucleocapsid Proteins - genetics</subject><subject>Nucleocapsid Proteins - metabolism</subject><subject>Nucleocapsids</subject><subject>Oncology</subject><subject>proteins</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Spodoptera</subject><subject>Sucrose</subject><subject>Ultracentrifugation</subject><subject>Virology</subject><subject>Virosomes - genetics</subject><subject>Virosomes - isolation & purification</subject><subject>Virosomes - metabolism</subject><subject>Virosomes - ultrastructure</subject><subject>Virus-like particles</subject><subject>viruses</subject><subject>Western blotting</subject><issn>1674-0769</issn><issn>1995-820X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkktv1DAUhS1ERcvAD2ADkZBglXL9iB8bJDSiFKlSkWAkdpaT2MElEw92Ujr8ehylTIEFrGzpHn_3nuOL0BMMpxhAvEqYkApKwLikBFTJ76ETrFRVSgKf7-c7F6wEwdUxepjSFQAnktIH6JhgJVQl6AnafIihnZrRh6EIrlivz8-Kax-nVPb-qy12Jo6-6W1R7wtT1KaZ-rCU_ZBsMxaN7fvC3uyiTWlmpH0a7fYROnKmT_bx7blCm7O3n9bn5cXlu_frNxdlwwTmJcsjiMpxSQyjQhnLCJNMGddwZq2pSRY4C60RlLcCBJWtI5JL7kxVKQZ0hV4v3N1Ub23b2GGMpte76Lcm7nUwXv9ZGfwX3YVrLUABYyQDXiyA72ZwZuj0VZjikEfWP7q6vSE5Wqhybln48rZTDN8mm0a99Wk2bwYbpqRnnFRQyf8qpcp-s--Z-fwv5aE7oUKCElUOaIXwompiSClad7CHQc9roJc10HlWPa-BnslPf8_l8OLXv2cBWQQpl4bOxrvW_6I-Wx45E7Tpok968zFHxCAnxAkA_Qm7V8Vu</recordid><startdate>201110</startdate><enddate>201110</enddate><creator>Zhou, Zhao-rui</creator><creator>Wang, Man-li</creator><creator>Deng, Fei</creator><creator>Li, Tian-xian</creator><creator>Hu, Zhi-hong</creator><creator>Wang, Hua-lin</creator><general>Springer-Verlag</general><general>SP Wuhan Institute of Virology, CAS</general><general>KeAi Publishing Communications Ltd</general><general>State Key Laboratory of Virology,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>201110</creationdate><title>Production of CCHF virus-like particle by a baculovirus-insect cell expression system</title><author>Zhou, Zhao-rui ; Wang, Man-li ; Deng, Fei ; Li, Tian-xian ; Hu, Zhi-hong ; Wang, Hua-lin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4716-457375f682a4379ae424849afc64eeab2457fe0da736d70738df28686fa559403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Baculoviridae</topic><topic>Baculoviridae - genetics</topic><topic>Baculovirus</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blotting, Western</topic><topic>Bunyaviridae</topic><topic>Cell Line</topic><topic>Centrifugation, Density Gradient - methods</topic><topic>Crimean hemorrhagic fever</topic><topic>Crimean-Congo hemorrhagic fever virus</topic><topic>Cytology</topic><topic>electron microscopes</topic><topic>Electron microscopy</topic><topic>Gene Expression</topic><topic>Genetic Vectors</topic><topic>Hemorrhagic Fever Virus, Crimean-Congo - genetics</topic><topic>Insect cells</topic><topic>insects</topic><topic>Medical Microbiology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>microscopy</topic><topic>Microscopy, Electron, Transmission</topic><topic>N protein</topic><topic>Nairovirus</topic><topic>nucleocapsid</topic><topic>Nucleocapsid Proteins - genetics</topic><topic>Nucleocapsid Proteins - metabolism</topic><topic>Nucleocapsids</topic><topic>Oncology</topic><topic>proteins</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Spodoptera</topic><topic>Sucrose</topic><topic>Ultracentrifugation</topic><topic>Virology</topic><topic>Virosomes - genetics</topic><topic>Virosomes - isolation & purification</topic><topic>Virosomes - metabolism</topic><topic>Virosomes - ultrastructure</topic><topic>Virus-like particles</topic><topic>viruses</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Zhao-rui</creatorcontrib><creatorcontrib>Wang, Man-li</creatorcontrib><creatorcontrib>Deng, Fei</creatorcontrib><creatorcontrib>Li, Tian-xian</creatorcontrib><creatorcontrib>Hu, Zhi-hong</creatorcontrib><creatorcontrib>Wang, Hua-lin</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Virologica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Zhao-rui</au><au>Wang, Man-li</au><au>Deng, Fei</au><au>Li, Tian-xian</au><au>Hu, Zhi-hong</au><au>Wang, Hua-lin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of CCHF virus-like particle by a baculovirus-insect cell expression system</atitle><jtitle>Virologica Sinica</jtitle><stitle>Virol. Sin</stitle><addtitle>Virol Sin</addtitle><date>2011-10</date><risdate>2011</risdate><volume>26</volume><issue>5</issue><spage>338</spage><epage>346</epage><pages>338-346</pages><artnum>338</artnum><issn>1674-0769</issn><eissn>1995-820X</eissn><abstract>Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope, Virus-Like Particles (VLPs) with various size and morphology were observed in cytoplasmic vesicles in the infected cells. Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation, which was confirmed by Western blot analysis and immuno-electron microscopy (IEM).</abstract><cop>Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21979573</pmid><doi>10.1007/s12250-011-3209-6</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1674-0769 |
| ispartof | Virologica Sinica, 2011-10, Vol.26 (5), p.338-346, Article 338 |
| issn | 1674-0769 1995-820X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7090442 |
| source | MEDLINE; PubMed Central; Alma/SFX Local Collection; SpringerLink Journals - AutoHoldings |
| subjects | Animals Baculoviridae Baculoviridae - genetics Baculovirus Biochemistry Biomedical and Life Sciences Biomedicine Blotting, Western Bunyaviridae Cell Line Centrifugation, Density Gradient - methods Crimean hemorrhagic fever Crimean-Congo hemorrhagic fever virus Cytology electron microscopes Electron microscopy Gene Expression Genetic Vectors Hemorrhagic Fever Virus, Crimean-Congo - genetics Insect cells insects Medical Microbiology Microbial Genetics and Genomics Microbiology microscopy Microscopy, Electron, Transmission N protein Nairovirus nucleocapsid Nucleocapsid Proteins - genetics Nucleocapsid Proteins - metabolism Nucleocapsids Oncology proteins Recombinant Proteins - genetics Recombinant Proteins - metabolism Spodoptera Sucrose Ultracentrifugation Virology Virosomes - genetics Virosomes - isolation & purification Virosomes - metabolism Virosomes - ultrastructure Virus-like particles viruses Western blotting |
| title | Production of CCHF virus-like particle by a baculovirus-insect cell expression system |
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