Sequence determination of the extreme 5' end of equine arteritis virus leader region
The extreme 5' end of the leader sequence of four equine arteritis virus (EAV) strains was obtained by using rapid amplification of cDNA end method (5' RACE), and sequenced. Seventeen more nucleotides were added upstream of the 5' end of the EAV published genomic sequence. A common fe...
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| Veröffentlicht in: | Virus genes 1996, Vol.12 (3), p.291-295 |
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| container_title | Virus genes |
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| creator | Kheyar, A. (Quebec Univ., Montreal, Que. (Canada). Dept. des Sciences Biologiques) St-Laurent, G Archambault, D |
| description | The extreme 5' end of the leader sequence of four equine arteritis virus (EAV) strains was obtained by using rapid amplification of cDNA end method (5' RACE), and sequenced. Seventeen more nucleotides were added upstream of the 5' end of the EAV published genomic sequence. A common feature among the analyzed EAV isolates was the presence of an AUG start codon within the added sequence and the appearance of an intraleader open reading frame (ORF) of 111 nucleotides which was predicted to encode a peptide of 37 amino acids. The role of this putative intraleader ORF has yet to be determined. |
| doi_str_mv | 10.1007/BF00284650 |
| format | Article |
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The role of this putative intraleader ORF has yet to be determined.</description><identifier>ISSN: 0920-8569</identifier><identifier>EISSN: 1572-994X</identifier><identifier>DOI: 10.1007/BF00284650</identifier><identifier>PMID: 8883367</identifier><language>eng</language><publisher>United States: Kluwer Academic Publishers</publisher><subject>ADN ; Alphaarterivirus equid ; amino acid sequences ; ARTERITE ; ARTERITIS ; Base Sequence ; chemistry ; codons ; DNA ; DNA, Viral ; DNA, Viral - chemistry ; Electrophoresis, Polyacrylamide Gel ; Equartevirus ; Equartevirus - genetics ; EQUIDAE ; equine arteritis virus ; genetics ; Molecular Sequence Data ; NUCLEOTIDE SEQUENCE ; Open Reading Frames ; RNA ; SECUENCIA NUCLEOTIDICA ; SEQUENCE NUCLEOTIDIQUE ; strains ; viral proteins ; VIROSE ; VIROSES ; VIROSIS</subject><ispartof>Virus genes, 1996, Vol.12 (3), p.291-295</ispartof><rights>Kluwer Academic Publishers 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-86fb8c9e4c277d93fac7c0d208a977258884bc3dfd575d5736d2a41267debf9c3</citedby><cites>FETCH-LOGICAL-c454t-86fb8c9e4c277d93fac7c0d208a977258884bc3dfd575d5736d2a41267debf9c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8883367$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kheyar, A. (Quebec Univ., Montreal, Que. (Canada). Dept. des Sciences Biologiques)</creatorcontrib><creatorcontrib>St-Laurent, G</creatorcontrib><creatorcontrib>Archambault, D</creatorcontrib><title>Sequence determination of the extreme 5' end of equine arteritis virus leader region</title><title>Virus genes</title><addtitle>Virus Genes</addtitle><description>The extreme 5' end of the leader sequence of four equine arteritis virus (EAV) strains was obtained by using rapid amplification of cDNA end method (5' RACE), and sequenced. Seventeen more nucleotides were added upstream of the 5' end of the EAV published genomic sequence. A common feature among the analyzed EAV isolates was the presence of an AUG start codon within the added sequence and the appearance of an intraleader open reading frame (ORF) of 111 nucleotides which was predicted to encode a peptide of 37 amino acids. 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Dept. des Sciences Biologiques)</au><au>St-Laurent, G</au><au>Archambault, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence determination of the extreme 5' end of equine arteritis virus leader region</atitle><jtitle>Virus genes</jtitle><addtitle>Virus Genes</addtitle><date>1996</date><risdate>1996</risdate><volume>12</volume><issue>3</issue><spage>291</spage><epage>295</epage><pages>291-295</pages><issn>0920-8569</issn><eissn>1572-994X</eissn><abstract>The extreme 5' end of the leader sequence of four equine arteritis virus (EAV) strains was obtained by using rapid amplification of cDNA end method (5' RACE), and sequenced. Seventeen more nucleotides were added upstream of the 5' end of the EAV published genomic sequence. A common feature among the analyzed EAV isolates was the presence of an AUG start codon within the added sequence and the appearance of an intraleader open reading frame (ORF) of 111 nucleotides which was predicted to encode a peptide of 37 amino acids. The role of this putative intraleader ORF has yet to be determined.</abstract><cop>United States</cop><pub>Kluwer Academic Publishers</pub><pmid>8883367</pmid><doi>10.1007/BF00284650</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0920-8569 |
| ispartof | Virus genes, 1996, Vol.12 (3), p.291-295 |
| issn | 0920-8569 1572-994X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7089268 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | ADN Alphaarterivirus equid amino acid sequences ARTERITE ARTERITIS Base Sequence chemistry codons DNA DNA, Viral DNA, Viral - chemistry Electrophoresis, Polyacrylamide Gel Equartevirus Equartevirus - genetics EQUIDAE equine arteritis virus genetics Molecular Sequence Data NUCLEOTIDE SEQUENCE Open Reading Frames RNA SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE strains viral proteins VIROSE VIROSES VIROSIS |
| title | Sequence determination of the extreme 5' end of equine arteritis virus leader region |
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