Sequence determination of the extreme 5' end of equine arteritis virus leader region

The extreme 5' end of the leader sequence of four equine arteritis virus (EAV) strains was obtained by using rapid amplification of cDNA end method (5' RACE), and sequenced. Seventeen more nucleotides were added upstream of the 5' end of the EAV published genomic sequence. A common fe...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Virus genes 1996, Vol.12 (3), p.291-295
Hauptverfasser:	Kheyar, A. (Quebec Univ., Montreal, Que. (Canada). Dept. des Sciences Biologiques), St-Laurent, G, Archambault, D
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN ARTERITE ARTERITIS Base Sequence DNA DNA, Viral - chemistry Electrophoresis, Polyacrylamide Gel Equartevirus - genetics EQUIDAE equine arteritis virus Molecular Sequence Data NUCLEOTIDE SEQUENCE Open Reading Frames SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE VIROSE VIROSES VIROSIS
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The extreme 5' end of the leader sequence of four equine arteritis virus (EAV) strains was obtained by using rapid amplification of cDNA end method (5' RACE), and sequenced. Seventeen more nucleotides were added upstream of the 5' end of the EAV published genomic sequence. A common feature among the analyzed EAV isolates was the presence of an AUG start codon within the added sequence and the appearance of an intraleader open reading frame (ORF) of 111 nucleotides which was predicted to encode a peptide of 37 amino acids. The role of this putative intraleader ORF has yet to be determined.
ISSN:	0920-8569 1572-994X
DOI:	10.1007/BF00284650