miR-18a Inhibitor Suppresses Leukemia Cell Proliferation by Upregulation of PTEN Expression

miR-18a Inhibitor Suppresses Leukemia Cell Proliferation by Upregulation of PTEN Expression

BACKGROUND Leukemia is common in aging adults and has very high mortality worldwide. The present study was designed to investigate the therapeutic efficacy of miR-18a inhibitor against WEHI-3 and THP-1 leukemia cells. MATERIAL AND METHODS The changes in miR-18a inhibitor-transfected WEHI-3 and THP-1...

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Veröffentlicht in:Medical science monitor 2020-03, Vol.26, p.e921288-e921288
Hauptverfasser: Zhang, Liping, Kong, Lingxia, Yang, Yuzhi
Format: Artikel
Sprache:eng
Schlagworte:
Apoptosis
Cell Proliferation - drug effects
Gene Expression Regulation, Leukemic
Humans
Lab/In Vitro Research
Leukemia - drug therapy
Leukemia - pathology
MicroRNAs - antagonists & inhibitors
PTEN Phosphohydrolase - metabolism
THP-1 Cells
Transfection
Up-Regulation
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Kong, Lingxia
Yang, Yuzhi
description BACKGROUND Leukemia is common in aging adults and has very high mortality worldwide. The present study was designed to investigate the therapeutic efficacy of miR-18a inhibitor against WEHI-3 and THP-1 leukemia cells. MATERIAL AND METHODS The changes in miR-18a inhibitor-transfected WEHI-3 and THP-1 cell proliferative potential was measured by use of the Cell Counting Kit-8 assay. Apoptotic changes were analyzed by electron microscopy, and evaluation of PI3K, AKT, mTOR, and PTEN expression was assessed by RT-qPCR assay. RESULTS Transfection of miR-18a inhibitor significantly (P
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The present study was designed to investigate the therapeutic efficacy of miR-18a inhibitor against WEHI-3 and THP-1 leukemia cells. MATERIAL AND METHODS The changes in miR-18a inhibitor-transfected WEHI-3 and THP-1 cell proliferative potential was measured by use of the Cell Counting Kit-8 assay. Apoptotic changes were analyzed by electron microscopy, and evaluation of PI3K, AKT, mTOR, and PTEN expression was assessed by RT-qPCR assay. RESULTS Transfection of miR-18a inhibitor significantly (P&lt;0.05) suppressed the proliferative potential of WEHI-3 and THP-1 cells. The WEHI-3 cells showed the presence of characteristic apoptotic bodies on transfection with miR-18a inhibitor at 48 h. Flow cytometry showed that miR-18a inhibitor transfection significantly (P&lt;0.05) increased the WEHI-3 cell percentage in G1 phase. The transfection of miR-18a inhibitor significantly (P&lt;0.05) promoted apoptosis in WEHI-3 cells. In WEHI-3 cells, miR-18a inhibitor transfection markedly suppressed the expression of PI3K, AKT, and mTOR mRNA. The expression of PTEN mRNA was significantly (P&lt;0.05) upregulated by miR-18a inhibitor transfection in WEHI-3 cells. CONCLUSIONS The present study investigated the therapeutic efficacy of miR-18a inhibitor against WEHI-3 and THP1 leukemia cells. The study demonstrated that miR-18a inhibitor suppressed the proliferative potential of WEHI-3 and THP1 cells and activated apoptotic process through upregulation of PTEN mRNA expression. Therefore, miR-18a inhibitor can be of therapeutic importance for the treatment of leukemia.</description><identifier>ISSN: 1643-3750</identifier><identifier>ISSN: 1234-1010</identifier><identifier>EISSN: 1643-3750</identifier><identifier>DOI: 10.12659/MSM.921288</identifier><identifier>PMID: 32146479</identifier><language>eng</language><publisher>United States: International Scientific Literature, Inc</publisher><subject>Apoptosis ; Cell Proliferation - drug effects ; Gene Expression Regulation, Leukemic ; Humans ; Lab/In Vitro Research ; Leukemia - drug therapy ; Leukemia - pathology ; MicroRNAs - antagonists &amp; inhibitors ; PTEN Phosphohydrolase - metabolism ; THP-1 Cells ; Transfection ; Up-Regulation</subject><ispartof>Medical science monitor, 2020-03, Vol.26, p.e921288-e921288</ispartof><rights>Med Sci Monit, 2020 2020</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-12a8f0a9c3264243d55a5ace4f3377400c87effe5a4a57463fd338cb783e9c683</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081926/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081926/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27922,27923,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32146479$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Liping</creatorcontrib><creatorcontrib>Kong, Lingxia</creatorcontrib><creatorcontrib>Yang, Yuzhi</creatorcontrib><title>miR-18a Inhibitor Suppresses Leukemia Cell Proliferation by Upregulation of PTEN Expression</title><title>Medical science monitor</title><addtitle>Med Sci Monit</addtitle><description>BACKGROUND Leukemia is common in aging adults and has very high mortality worldwide. The present study was designed to investigate the therapeutic efficacy of miR-18a inhibitor against WEHI-3 and THP-1 leukemia cells. MATERIAL AND METHODS The changes in miR-18a inhibitor-transfected WEHI-3 and THP-1 cell proliferative potential was measured by use of the Cell Counting Kit-8 assay. Apoptotic changes were analyzed by electron microscopy, and evaluation of PI3K, AKT, mTOR, and PTEN expression was assessed by RT-qPCR assay. RESULTS Transfection of miR-18a inhibitor significantly (P&lt;0.05) suppressed the proliferative potential of WEHI-3 and THP-1 cells. The WEHI-3 cells showed the presence of characteristic apoptotic bodies on transfection with miR-18a inhibitor at 48 h. Flow cytometry showed that miR-18a inhibitor transfection significantly (P&lt;0.05) increased the WEHI-3 cell percentage in G1 phase. The transfection of miR-18a inhibitor significantly (P&lt;0.05) promoted apoptosis in WEHI-3 cells. In WEHI-3 cells, miR-18a inhibitor transfection markedly suppressed the expression of PI3K, AKT, and mTOR mRNA. The expression of PTEN mRNA was significantly (P&lt;0.05) upregulated by miR-18a inhibitor transfection in WEHI-3 cells. CONCLUSIONS The present study investigated the therapeutic efficacy of miR-18a inhibitor against WEHI-3 and THP1 leukemia cells. The study demonstrated that miR-18a inhibitor suppressed the proliferative potential of WEHI-3 and THP1 cells and activated apoptotic process through upregulation of PTEN mRNA expression. Therefore, miR-18a inhibitor can be of therapeutic importance for the treatment of leukemia.</description><subject>Apoptosis</subject><subject>Cell Proliferation - drug effects</subject><subject>Gene Expression Regulation, Leukemic</subject><subject>Humans</subject><subject>Lab/In Vitro Research</subject><subject>Leukemia - drug therapy</subject><subject>Leukemia - pathology</subject><subject>MicroRNAs - antagonists &amp; inhibitors</subject><subject>PTEN Phosphohydrolase - metabolism</subject><subject>THP-1 Cells</subject><subject>Transfection</subject><subject>Up-Regulation</subject><issn>1643-3750</issn><issn>1234-1010</issn><issn>1643-3750</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkT1PwzAQhi0EgvIxsSOPSCjFn4mzIKGqQKUCFW0nBstxbTAkcbETBP-eiAAq0_nOj9873wvAMUZDTFKen9_Ob4c5wUSILTDAKaMJzTja3jjvgf0YXxAiIkV8F-xRglnKsnwAHiv3kGCh4KR-doVrfIDzdr0OJkYT4dS0r6ZyCo5MWcJZ8KWzJqjG-RoWn3DZcU9t2efewtlifAfHH9-vu9Ih2LGqjOboJx6A5dV4MbpJpvfXk9HlNNGM0CbBRAmLVK4pSRlhdMW54kobZinNMoaQFpmx1nDFFM9YSu2KUqGLTFCT61TQA3DR667bojIrbeomqFKug6tU-JReOfn_pnbP8sm_ywwJnJO0Ezj9EQj-rTWxkZWLuvuyqo1voyTdDjlGnOUdetajOvgYg7F_bTCS33bIzg7Z29HRJ5uT_bG_-6dfo76GZQ</recordid><startdate>20200308</startdate><enddate>20200308</enddate><creator>Zhang, Liping</creator><creator>Kong, Lingxia</creator><creator>Yang, Yuzhi</creator><general>International Scientific Literature, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200308</creationdate><title>miR-18a Inhibitor Suppresses Leukemia Cell Proliferation by Upregulation of PTEN Expression</title><author>Zhang, Liping ; Kong, Lingxia ; Yang, Yuzhi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-12a8f0a9c3264243d55a5ace4f3377400c87effe5a4a57463fd338cb783e9c683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Apoptosis</topic><topic>Cell Proliferation - drug effects</topic><topic>Gene Expression Regulation, Leukemic</topic><topic>Humans</topic><topic>Lab/In Vitro Research</topic><topic>Leukemia - drug therapy</topic><topic>Leukemia - pathology</topic><topic>MicroRNAs - antagonists &amp; inhibitors</topic><topic>PTEN Phosphohydrolase - metabolism</topic><topic>THP-1 Cells</topic><topic>Transfection</topic><topic>Up-Regulation</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Liping</creatorcontrib><creatorcontrib>Kong, Lingxia</creatorcontrib><creatorcontrib>Yang, Yuzhi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Medical science monitor</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Liping</au><au>Kong, Lingxia</au><au>Yang, Yuzhi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>miR-18a Inhibitor Suppresses Leukemia Cell Proliferation by Upregulation of PTEN Expression</atitle><jtitle>Medical science monitor</jtitle><addtitle>Med Sci Monit</addtitle><date>2020-03-08</date><risdate>2020</risdate><volume>26</volume><spage>e921288</spage><epage>e921288</epage><pages>e921288-e921288</pages><issn>1643-3750</issn><issn>1234-1010</issn><eissn>1643-3750</eissn><abstract>BACKGROUND Leukemia is common in aging adults and has very high mortality worldwide. The present study was designed to investigate the therapeutic efficacy of miR-18a inhibitor against WEHI-3 and THP-1 leukemia cells. MATERIAL AND METHODS The changes in miR-18a inhibitor-transfected WEHI-3 and THP-1 cell proliferative potential was measured by use of the Cell Counting Kit-8 assay. Apoptotic changes were analyzed by electron microscopy, and evaluation of PI3K, AKT, mTOR, and PTEN expression was assessed by RT-qPCR assay. RESULTS Transfection of miR-18a inhibitor significantly (P&lt;0.05) suppressed the proliferative potential of WEHI-3 and THP-1 cells. The WEHI-3 cells showed the presence of characteristic apoptotic bodies on transfection with miR-18a inhibitor at 48 h. Flow cytometry showed that miR-18a inhibitor transfection significantly (P&lt;0.05) increased the WEHI-3 cell percentage in G1 phase. The transfection of miR-18a inhibitor significantly (P&lt;0.05) promoted apoptosis in WEHI-3 cells. In WEHI-3 cells, miR-18a inhibitor transfection markedly suppressed the expression of PI3K, AKT, and mTOR mRNA. The expression of PTEN mRNA was significantly (P&lt;0.05) upregulated by miR-18a inhibitor transfection in WEHI-3 cells. CONCLUSIONS The present study investigated the therapeutic efficacy of miR-18a inhibitor against WEHI-3 and THP1 leukemia cells. The study demonstrated that miR-18a inhibitor suppressed the proliferative potential of WEHI-3 and THP1 cells and activated apoptotic process through upregulation of PTEN mRNA expression. Therefore, miR-18a inhibitor can be of therapeutic importance for the treatment of leukemia.</abstract><cop>United States</cop><pub>International Scientific Literature, Inc</pub><pmid>32146479</pmid><doi>10.12659/MSM.921288</doi><oa>free_for_read</oa></addata></record>
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subjects Apoptosis
Cell Proliferation - drug effects
Gene Expression Regulation, Leukemic
Humans
Lab/In Vitro Research
Leukemia - drug therapy
Leukemia - pathology
MicroRNAs - antagonists & inhibitors
PTEN Phosphohydrolase - metabolism
THP-1 Cells
Transfection
Up-Regulation
title miR-18a Inhibitor Suppresses Leukemia Cell Proliferation by Upregulation of PTEN Expression
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