miR-18a Inhibitor Suppresses Leukemia Cell Proliferation by Upregulation of PTEN Expression

BACKGROUND Leukemia is common in aging adults and has very high mortality worldwide. The present study was designed to investigate the therapeutic efficacy of miR-18a inhibitor against WEHI-3 and THP-1 leukemia cells. MATERIAL AND METHODS The changes in miR-18a inhibitor-transfected WEHI-3 and THP-1...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Medical science monitor 2020-03, Vol.26, p.e921288-e921288
Hauptverfasser:	Zhang, Liping, Kong, Lingxia, Yang, Yuzhi
Format:	Artikel
Sprache:	eng
Schlagworte:	Apoptosis Cell Proliferation - drug effects Gene Expression Regulation, Leukemic Humans Lab/In Vitro Research Leukemia - drug therapy Leukemia - pathology MicroRNAs - antagonists & inhibitors PTEN Phosphohydrolase - metabolism THP-1 Cells Transfection Up-Regulation
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	BACKGROUND Leukemia is common in aging adults and has very high mortality worldwide. The present study was designed to investigate the therapeutic efficacy of miR-18a inhibitor against WEHI-3 and THP-1 leukemia cells. MATERIAL AND METHODS The changes in miR-18a inhibitor-transfected WEHI-3 and THP-1 cell proliferative potential was measured by use of the Cell Counting Kit-8 assay. Apoptotic changes were analyzed by electron microscopy, and evaluation of PI3K, AKT, mTOR, and PTEN expression was assessed by RT-qPCR assay. RESULTS Transfection of miR-18a inhibitor significantly (P
ISSN:	1643-3750 1234-1010 1643-3750
DOI:	10.12659/MSM.921288