Multi-functionPlantDefensin,AntimicrobialandHeavyMetal Adsorbent Peptide

Defensin peptide isolated from plants are often heterogeneous in length, sequence and structure, but they are mostly small, cationic and amphipathic. Plant defensins exhibit broad-spectrum antibacterial and antifungal activities against Gram-positive and Gram-negative bacteria, fungi and etc. Plant defensins also play an important role in innate immunity, such as heavy metal and some abiotic stresses tolerance. In this paper, broad-spectrum activities, antimicrobial and heavy metal absorption, of a recombinant plant defensin were studied. gene, a modified plant defensin gene, was cloned in pBISN1-IN (EU886197) plasmid, recombinant protein was produced by transient expression via Agroinfiltration method in common bean. The recombinant protein was tested for antibacterial activity against Gram-negative, Gram-positive bacteria and Fusarium sp. the effects of different treatments on heavy metal zinc absorption by this peptide were tested. We confirmed the antibacterial activities of this peptide against Gram-negative ( and ) and Gram-positive ( and ) bacteria, and antifungal activities of this peptide against Fusarium spp. ( and ). High metal absorption coefficient for this peptide was also observed. Out of six actinobacterial isolates, VITVAMB 1 possessed the most efficient RO-16 decolorization property. It decolorized 85.6% of RO-16 (250 mg L ) within 24hrs. Isolate VITVAMB 1 was identified to be sp. Maximum dye decolorization occurred at pH 8, temperature 35oC, 3% salt concentration and a dye concentration of 50 mg L . Results suggesting that modified defensin peptide facilitates a broader range of defense activities. dedefensins are an important part of the innate immune system in eukaryotes. These molecules have multidimensional properties that making them promising agents for therapeutic drugs.