A novel test for determination of wild felid-domestic cat hybridization

[Display omitted] •Felis catus genomes harbor multiple copies of endogenous feline leukemia virus (enFeLV)•Lynx rufus genomes do not harbor enFeLV•F. catus and L. rufus hybrids would have at least 1 copy enFeLV per cell•The contested animal lacks any enFeLV•The contested animal is not a domestic cat hybrid In October 2018, Colorado Parks and Wildlife seized an animal believed to be an illegally possessed bobcat. The owner claimed the animal was a bobcat/domestic cat hybrid, exempted from license requirements. Burden of proof lay with CPW to determine the lineage of the animal. Commercial microsatellite arrays and DNA barcoding have not been developed for identification of bobcat/domestic cat hybrids, and limited time and resources prevented development of such tests for this application. Instead, we targeted endogenous feline leukemia virus (enFeLV) to quickly and inexpensively demonstrate the absence of domestic cat DNA in the contested animal. Using this assay, we were able to confirm that the contested animal lacked enFeLV, and therefore was not a domestic cat hybrid.