In vitro response and gene expression of human retinal Müller cells treated with different anti-VEGF drugs

•Human retinal Müller cells (MIO-M1) were treated with 4 different anti-VEGF drugs.•Cultures had decreased metabolism, viability and mitochondrial membrane potential.•Treatment led to higher levels of ROS and apoptosis.•Altered expression of angiogenesis, pro-apoptosis and inflammatory genes were fo...

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Veröffentlicht in:	Experimental eye research 2020-02, Vol.191, p.107903-107903, Article 107903
Hauptverfasser:	Cáceres-del-Carpio, Javier, Moustafa, M. Tarek, Toledo-Corral, Jaime, Hamid, Mohamed A., Atilano, Shari R., Schneider, Kevin, Fukuhara, Paula S., Costa, Rodrigo Donato, Norman, J. Lucas, Malik, Deepika, Chwa, Marilyn, Boyer, David S., Limb, G. Astrid, Kenney, M. Cristina, Kuppermann, Baruch D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins - genetics Angiogenesis Inhibitors - pharmacology Angiogenic Proteins - genetics Apoptosis Apoptosis Regulatory Proteins - genetics Bevacizumab - pharmacology Carrier Proteins - genetics Cell Line Cell Survival Ependymoglial Cells - drug effects Ependymoglial Cells - metabolism Gene Expression - physiology Glial Fibrillary Acidic Protein - genetics Humans Inflammation - genetics Membrane Potential, Mitochondrial - physiology Oxidative Stress - genetics Ranibizumab - pharmacology Reactive Oxygen Species - metabolism Real-Time Polymerase Chain Reaction Receptors, Vascular Endothelial Growth Factor Recombinant Fusion Proteins - pharmacology Vascular Endothelial Growth Factor A - antagonists & inhibitors
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Zusammenfassung:	•Human retinal Müller cells (MIO-M1) were treated with 4 different anti-VEGF drugs.•Cultures had decreased metabolism, viability and mitochondrial membrane potential.•Treatment led to higher levels of ROS and apoptosis.•Altered expression of angiogenesis, pro-apoptosis and inflammatory genes were found.•Anti-VEGF drugs may contribute to cytotoxicity associated with chronic injections.
ISSN:	0014-4835 1096-0007 1096-0007
DOI:	10.1016/j.exer.2019.107903