Concentration Determination of >200 Proteins in Dried Blood Spots for Biomarker Discovery and Validation
Multiple reaction monitoring with isotope dilution assays were developed for quantification of 200 proteins in dried blood spots, with median inter-day CV of 8%. Developed assays were used to measure the concentration ranges of these 200 proteins in twenty same sex, same race and age matched individ...
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Veröffentlicht in: | Molecular & cellular proteomics 2020-03, Vol.19 (3), p.540-553 |
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Zusammenfassung: | Multiple reaction monitoring with isotope dilution assays were developed for quantification of 200 proteins in dried blood spots, with median inter-day CV of 8%. Developed assays were used to measure the concentration ranges of these 200 proteins in twenty same sex, same race and age matched individuals. These, precise, sensitive, and multiplexed assays can be applied for de novo biomarker discovery and for biomarker quantification or verification experiments.
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Highlights
•Repeatable quantification of 200 proteins in dried blood spots.•Determined lower limit of quantification, repeatability, parallelism and stability.•Protein stability in DBS stored at ambient temperatures for up to 2 months.•Concentration ranges for 200 proteins in 20 healthy individuals.
The use of protein biomarkers as surrogates for clinical endpoints requires extensive multilevel validation including development of robust and sensitive assays for precise measurement of protein concentration. Multiple reaction monitoring (MRM) is a well-established mass-spectrometric method that can be used for reproducible protein-concentration measurements in biological specimens collected via microsampling. The dried blood spot (DBS) microsampling technique can be performed non-invasively without the expertise of a phlebotomist, and can enhance analyte stability which facilitate the application of this technique in retrospective studies while providing lower storage and shipping costs, because cold-chain logistics can be eliminated. Thus, precise, sensitive, and multiplexed methods for measuring protein concentrations in DBSs can be used for de novo biomarker discovery and for biomarker quantification or verification experiments. To achieve this goal, MRM assays were developed for multiplexed concentration measurement of proteins in DBSs.
The lower limit of quantification (LLOQ) was found to have a median total coefficient of variation (CV) of 18% for 245 proteins, whereas the median LLOQ was 5 fmol of peptide injected on column, and the median inter-day CV over 4 days for measuring endogenous protein concentration was 8%. The majority (88%) of the assays displayed parallelism, whereas the peptide standards remained stable throughout the assay workflow and after exposure to multiple freeze-thaw cycles. For 190 proteins, the measured protein concentrations remained stable in DBS stored at ambient laboratory temperature for up to 2 months. Finally, the developed assays were used to me |
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ISSN: | 1535-9476 1535-9484 |
DOI: | 10.1074/mcp.TIR119.001820 |