Development of membrane-based flow-through assay for detection of trypanosomosis in equines
A rapid flow-through immunoassay using protein A labeled gold nanoparticles (GNPs) for the qualitative detection of trypanosomosis in equine serum samples was developed. The flow-through device consisted of antigen-coated nitrocellulose membrane fixed on absorbent pads. The GNPs based detection prob...
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| Veröffentlicht in: | Journal of parasitic diseases 2020-03, Vol.44 (1), p.99-104 |
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| container_title | Journal of parasitic diseases |
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| creator | Kumar, Ritesh Yadav, Suresh Chandra Kumar, Sandeep Dilbaghi, Neeraj |
| description | A rapid flow-through immunoassay using protein A labeled gold nanoparticles (GNPs) for the qualitative detection of trypanosomosis in equine serum samples was developed. The flow-through device consisted of antigen-coated nitrocellulose membrane fixed on absorbent pads. The GNPs based detection probe was used as the colored marker. The assay can be employed for the detection of
Trypanosoma
specific antibodies at 14 days post-infection in the infected pooled serum samples of equines. The assay can be conducted in |
| doi_str_mv | 10.1007/s12639-019-01166-8 |
| format | Article |
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Trypanosoma
specific antibodies at 14 days post-infection in the infected pooled serum samples of equines. The assay can be conducted in < 5 min. The results of the flow-through assay were comparable with the methods like ELISA, dot blot assay and lateral flow assay. The developed assay showed high efficiency for detection of anti-
Trypanosoma
antibodies in equine sera and can be used for quick screening of serum samples under field conditions.</description><identifier>ISSN: 0971-7196</identifier><identifier>EISSN: 0975-0703</identifier><identifier>DOI: 10.1007/s12639-019-01166-8</identifier><identifier>PMID: 32174710</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Antibodies ; Enzyme-linked immunosorbent assay ; Health Promotion and Disease Prevention ; Infectious Diseases ; Medicine ; Medicine & Public Health ; Nanoparticles ; Original ; Original Article ; Trypanosoma</subject><ispartof>Journal of parasitic diseases, 2020-03, Vol.44 (1), p.99-104</ispartof><rights>Indian Society for Parasitology 2019</rights><rights>Indian Society for Parasitology 2019.</rights><rights>2019© Indian Society for Parasitology 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3898-4989e38e30fa53c7e64f28ced2a5ec25247cc2cd25537da641c462176a3856d83</citedby><cites>FETCH-LOGICAL-c3898-4989e38e30fa53c7e64f28ced2a5ec25247cc2cd25537da641c462176a3856d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046882/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046882/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32174710$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kumar, Ritesh</creatorcontrib><creatorcontrib>Yadav, Suresh Chandra</creatorcontrib><creatorcontrib>Kumar, Sandeep</creatorcontrib><creatorcontrib>Dilbaghi, Neeraj</creatorcontrib><title>Development of membrane-based flow-through assay for detection of trypanosomosis in equines</title><title>Journal of parasitic diseases</title><addtitle>J Parasit Dis</addtitle><addtitle>J Parasit Dis</addtitle><description>A rapid flow-through immunoassay using protein A labeled gold nanoparticles (GNPs) for the qualitative detection of trypanosomosis in equine serum samples was developed. The flow-through device consisted of antigen-coated nitrocellulose membrane fixed on absorbent pads. The GNPs based detection probe was used as the colored marker. The assay can be employed for the detection of
Trypanosoma
specific antibodies at 14 days post-infection in the infected pooled serum samples of equines. The assay can be conducted in < 5 min. The results of the flow-through assay were comparable with the methods like ELISA, dot blot assay and lateral flow assay. The developed assay showed high efficiency for detection of anti-
Trypanosoma
antibodies in equine sera and can be used for quick screening of serum samples under field conditions.</description><subject>Antibodies</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Health Promotion and Disease Prevention</subject><subject>Infectious Diseases</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Nanoparticles</subject><subject>Original</subject><subject>Original Article</subject><subject>Trypanosoma</subject><issn>0971-7196</issn><issn>0975-0703</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kUtv1DAUha0KRNuBP8CiitQNG4Mf8SObSlWhgFSJDV11YXmcm5lUiT31TVrNv8fTKS2wQJZly_e7xz4-hLzn7CNnzHxCLrRsKOO7ybWm9oAcscYoygyTrx73nBre6ENyjHjLmCrn9g05lIKb2nB2RG4-wz0MaTNCnKrUVSOMy-wj0KVHaKtuSA90Wuc0r9aVR_Tbqku5amGCMPUp7lqmvN34mDCNCXus-ljB3dxHwLfkdecHhHdP64JcX375efGNXv34-v3i_IoGaRtL68Y2IC1I1nklgwFdd8IGaIVXEIQStQlBhFYoJU3rdc1DrYsB7aVVurVyQc72upt5OUIbipXsB7fJ_ejz1iXfu78rsV-7Vbp3htXaWlEEPjwJ5HQ3A05u7DHAMJSPSDM6IY3RVvIyFuT0H_Q2zTkWe4XS2jIlmSyU2FMhJ8QM3fNjOHO77Nw-O1eyc4_ZuZ2Nkz9tPLf8DqsAcg9gKcUV5Je7_yP7C1Jhpes</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Kumar, Ritesh</creator><creator>Yadav, Suresh Chandra</creator><creator>Kumar, Sandeep</creator><creator>Dilbaghi, Neeraj</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200301</creationdate><title>Development of membrane-based flow-through assay for detection of trypanosomosis in equines</title><author>Kumar, Ritesh ; Yadav, Suresh Chandra ; Kumar, Sandeep ; Dilbaghi, Neeraj</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3898-4989e38e30fa53c7e64f28ced2a5ec25247cc2cd25537da641c462176a3856d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antibodies</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Health Promotion and Disease Prevention</topic><topic>Infectious Diseases</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Nanoparticles</topic><topic>Original</topic><topic>Original Article</topic><topic>Trypanosoma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumar, Ritesh</creatorcontrib><creatorcontrib>Yadav, Suresh Chandra</creatorcontrib><creatorcontrib>Kumar, Sandeep</creatorcontrib><creatorcontrib>Dilbaghi, Neeraj</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of parasitic diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumar, Ritesh</au><au>Yadav, Suresh Chandra</au><au>Kumar, Sandeep</au><au>Dilbaghi, Neeraj</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of membrane-based flow-through assay for detection of trypanosomosis in equines</atitle><jtitle>Journal of parasitic diseases</jtitle><stitle>J Parasit Dis</stitle><addtitle>J Parasit Dis</addtitle><date>2020-03-01</date><risdate>2020</risdate><volume>44</volume><issue>1</issue><spage>99</spage><epage>104</epage><pages>99-104</pages><issn>0971-7196</issn><eissn>0975-0703</eissn><abstract>A rapid flow-through immunoassay using protein A labeled gold nanoparticles (GNPs) for the qualitative detection of trypanosomosis in equine serum samples was developed. The flow-through device consisted of antigen-coated nitrocellulose membrane fixed on absorbent pads. The GNPs based detection probe was used as the colored marker. The assay can be employed for the detection of
Trypanosoma
specific antibodies at 14 days post-infection in the infected pooled serum samples of equines. The assay can be conducted in < 5 min. The results of the flow-through assay were comparable with the methods like ELISA, dot blot assay and lateral flow assay. The developed assay showed high efficiency for detection of anti-
Trypanosoma
antibodies in equine sera and can be used for quick screening of serum samples under field conditions.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>32174710</pmid><doi>10.1007/s12639-019-01166-8</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; SpringerLink Journals - AutoHoldings |
| subjects | Antibodies Enzyme-linked immunosorbent assay Health Promotion and Disease Prevention Infectious Diseases Medicine Medicine & Public Health Nanoparticles Original Original Article Trypanosoma |
| title | Development of membrane-based flow-through assay for detection of trypanosomosis in equines |
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