Development of membrane-based flow-through assay for detection of trypanosomosis in equines

A rapid flow-through immunoassay using protein A labeled gold nanoparticles (GNPs) for the qualitative detection of trypanosomosis in equine serum samples was developed. The flow-through device consisted of antigen-coated nitrocellulose membrane fixed on absorbent pads. The GNPs based detection prob...

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Veröffentlicht in:	Journal of parasitic diseases 2020-03, Vol.44 (1), p.99-104
Hauptverfasser:	Kumar, Ritesh, Yadav, Suresh Chandra, Kumar, Sandeep, Dilbaghi, Neeraj
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies Enzyme-linked immunosorbent assay Health Promotion and Disease Prevention Infectious Diseases Medicine Medicine & Public Health Nanoparticles Original Original Article Trypanosoma
Online-Zugang:	Volltext
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Zusammenfassung:	A rapid flow-through immunoassay using protein A labeled gold nanoparticles (GNPs) for the qualitative detection of trypanosomosis in equine serum samples was developed. The flow-through device consisted of antigen-coated nitrocellulose membrane fixed on absorbent pads. The GNPs based detection probe was used as the colored marker. The assay can be employed for the detection of Trypanosoma specific antibodies at 14 days post-infection in the infected pooled serum samples of equines. The assay can be conducted in
ISSN:	0971-7196 0975-0703
DOI:	10.1007/s12639-019-01166-8