Pullulan-based dissolving microneedle arrays for enhanced transdermal delivery of small and large biomolecules

One specific technological advance in transdermal drug delivery is the development of dissolving microneedles (DMNs), which efficiently deliver therapeutics through a rapid dissolution of polymers after penetration into the skin. However, there is a limited range of water soluble, biodegradable polymers that can be used to manufacture DMN. Here, we report for the first time, the preparation and characterisation of a DMN system from the carbohydrate biopolymer, pullulan (PL). PL gels, of varying concentration, were studied for viscosity, film formation properties, and subsequently, microneedle formation. Model molecules and protein/peptide were loaded into PL DMN and characterised. The stability of model biomolecules, such as FITC-BSA and insulin, following DMN manufacture were assessed using circular dichroism. Ex-vivo porcine skin permeation studies using Franz diffusion cell apparatus for Flu-Na and FITC-BSA loaded PL-DMN were conducted. This study demonstrates that PL DMNs may serve as a promising tool for efficient transdermal drug delivery. [Display omitted] •Pullulan microneedles deliver both high and low molecular weight molecules across skin in vitro.•Biomolecules were stable in gels used to prepare pullulan microneedles.•Pulluan microneedles exhibited promising physical properties.•Pullulan microneedles may be useful in transdermal delivery of biomolecules.