Crosstalk between DNA methylation and gene expression in colorectal cancer, a potential plasma biomarker for tracing this tumor

Colorectal cancer (CRC), the second leading cause of cancer mortality, constitutes a significant global health burden. An accurate, noninvasive detection method for CRC as complement to colonoscopy could improve the effectiveness of treatment. In the present study, SureSelectXT Methyl-Seq was perfor...

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Veröffentlicht in:	Scientific reports 2020-02, Vol.10 (1), p.2813-2813, Article 2813
Hauptverfasser:	Kerachian, Mohammad Amin, Javadmanesh, Ali, Azghandi, Marjan, Mojtabanezhad Shariatpanahi, Afsaneh, Yassi, Maryam, Shams Davodly, Ehsan, Talebi, Amin, Khadangi, Fatemeh, Soltani, Ghodratollah, Hayatbakhsh, Abdorasool, Ghaffarzadegan, Kamran
Format:	Artikel
Sprache:	eng
Schlagworte:	38/22 38/23 38/47 38/77 38/90 631/67/68/2486 692/4028/67/68/2486 Adenocarcinoma - genetics Adult Aged Aged, 80 and over Biomarkers Biomarkers, Tumor - blood Cancer Cell-Free Nucleic Acids - blood Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - genetics Deoxyribonucleic acid DNA DNA Methylation Female Gene expression Global health Humanities and Social Sciences Humans Male Middle Aged multidisciplinary Overexpression Paraffin Pilot Projects Science Science (multidisciplinary) Tissues Young Adult
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Zusammenfassung:	Colorectal cancer (CRC), the second leading cause of cancer mortality, constitutes a significant global health burden. An accurate, noninvasive detection method for CRC as complement to colonoscopy could improve the effectiveness of treatment. In the present study, SureSelectXT Methyl-Seq was performed on cancerous and normal colon tissues and CLDN1 , INHBA and SLC30A10 were found as candidate methylated genes. MethyLight assay was run on formalin-fixed paraffin-embedded (FFPE) and fresh case and control tissues to validate the methylation of the selected gene. The methylation was significantly different (p-values
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-020-59690-0