Pressure sensing through Piezo channels controls whether cells migrate with blebs or pseudopods

Blebs and pseudopods can both power cell migration, with blebs often favored in tissues, where cells encounter increased mechanical resistance. To investigate how migrating cells detect and respond to mechanical forces, we used a “cell squasher” to apply uniaxial pressure to Dictyostelium cells chem...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2020-02, Vol.117 (5), p.2506-2512
Hauptverfasser:	Srivastava, Nishit, Traynor, David, Piel, Matthieu, Kabla, Alexandre J., Kay, Robert R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Actin Biological Sciences Biomechanical Phenomena Calcium Calcium (extracellular) Calcium influx Cell adhesion & migration Cell Behavior Cell migration Cell Movement Cellular Biology Chemotaxis Cytoplasm Cytoplasm - chemistry Cytoplasm - genetics Cytoplasm - metabolism Cytoskeleton Dictyostelium - chemistry Dictyostelium - cytology Dictyostelium - genetics Dictyostelium - metabolism Ion Channels - genetics Ion Channels - metabolism Life Sciences Mechanotransduction, Cellular Myosin Myosin Type II - genetics Myosin Type II - metabolism PNAS Plus Pressure Protozoan Proteins - genetics Protozoan Proteins - metabolism Pseudopodia - genetics Pseudopodia - metabolism Subcellular Processes
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Zusammenfassung:	Blebs and pseudopods can both power cell migration, with blebs often favored in tissues, where cells encounter increased mechanical resistance. To investigate how migrating cells detect and respond to mechanical forces, we used a “cell squasher” to apply uniaxial pressure to Dictyostelium cells chemotaxing under soft agarose. As little as 100 Pa causes a rapid (
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.1905730117