The impact of lncRNA MG3 on laryngeal cancer cell growth, cycle, and apoptosis related factors

Laryngeal cancer is a common head and neck malignant tumor. Long non-coding RNA (lncRNA) is a kind of RNA at the length more than 200 nucleotides that cannot code protein. They widely exist in the human genome. Maternally expressed gene 3 (MG3) is a kind of lncRNA that is associated with various mal...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:International journal of clinical and experimental pathology 2017-01, Vol.10 (7), p.7475-7480
Hauptverfasser: Li, Yuan, Zhou, Xuehua, Tao, Chenjuan, Chen, Chaohui, Cui, Caixia, Dai, Lili, Wu, Honglin, Wei, Qingyu, Luo, Song
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 7480
container_issue 7
container_start_page 7475
container_title International journal of clinical and experimental pathology
container_volume 10
creator Li, Yuan
Zhou, Xuehua
Tao, Chenjuan
Chen, Chaohui
Cui, Caixia
Dai, Lili
Wu, Honglin
Wei, Qingyu
Luo, Song
description Laryngeal cancer is a common head and neck malignant tumor. Long non-coding RNA (lncRNA) is a kind of RNA at the length more than 200 nucleotides that cannot code protein. They widely exist in the human genome. Maternally expressed gene 3 (MG3) is a kind of lncRNA that is associated with various malignant tumors development. This study explores the influence of MG3 on laryngeal cancer Hep-2 cell growth, cycle, and apoptosis related factors. Hep2 cells transfected by MG3 were treated as experimental group, while untransfected Hep2 cells were treated as control. MG3 expression was tested by real time PCR. Cell proliferation was evaluated by MTT assay. Cell cycle was determined by flow cytometry. Bcl-2, Bax, and survivin protein levels were detected by Western blot. MG3 expression significantly increased in Hep2 cells compared with control (P < 0.05). Hep2 cell OD value and cell percentage in S phase were obviously declined, while cell apoptosis were markedly enhanced (P < 0.05). OD value and cell percentage in S phase apparently reduced in 12 h, 24 h, and 48 h from experimental group (P < 0.05). Bcl-2 and survivin protein downregulated, while Bax protein elevated in experimental group following time extension (P < 0.05). MG3 overexpression inhibited laryngeal cancer Hep2 cell proliferation and arrested cell cycle with time dependence, which may achieve by suppressing Bcl-2 and survivin protein, and facilitating Bax protein expression.
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6965295</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2343509698</sourcerecordid><originalsourceid>FETCH-LOGICAL-p266t-d7266e5c8094605f39936ce65bc467ea23be5132701c24895fcab5b61a1297e33</originalsourceid><addsrcrecordid>eNpVkE9LAzEUxBdBbK1-BcnRQxfyZ5NtLkIpWoWqIPVqyGbftivZZE1Spd_eFavoaQ5v-M3MO8rGRDKRU0H5KDuN8RVjQWiBT7IRI1IILsk4e1lvAbVdr01CvkHWmaeHObpfMuQdsjrs3Qa0RUY7AwEZsBZtgv9I2ykye2NhirSrke59n3xsIwpgdYIaNQPQh3iWHTfaRjg_6CR7vrleL27z1ePybjFf5T0VIuV1OQhwM8OyEJg3TA7NDQhemUKUoCmrgBNGS0wMLWaSN0ZXvBJEEypLYGySXX1z-13VQW3ApaCt6kPbDRuU1636f3HtVm38uxJScCr5ALg8AIJ_20FMqmvj11ztwO-ioqxgHEshZ4P14m_Wb8jPU9knnftyYw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2343509698</pqid></control><display><type>article</type><title>The impact of lncRNA MG3 on laryngeal cancer cell growth, cycle, and apoptosis related factors</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Li, Yuan ; Zhou, Xuehua ; Tao, Chenjuan ; Chen, Chaohui ; Cui, Caixia ; Dai, Lili ; Wu, Honglin ; Wei, Qingyu ; Luo, Song</creator><creatorcontrib>Li, Yuan ; Zhou, Xuehua ; Tao, Chenjuan ; Chen, Chaohui ; Cui, Caixia ; Dai, Lili ; Wu, Honglin ; Wei, Qingyu ; Luo, Song</creatorcontrib><description>Laryngeal cancer is a common head and neck malignant tumor. Long non-coding RNA (lncRNA) is a kind of RNA at the length more than 200 nucleotides that cannot code protein. They widely exist in the human genome. Maternally expressed gene 3 (MG3) is a kind of lncRNA that is associated with various malignant tumors development. This study explores the influence of MG3 on laryngeal cancer Hep-2 cell growth, cycle, and apoptosis related factors. Hep2 cells transfected by MG3 were treated as experimental group, while untransfected Hep2 cells were treated as control. MG3 expression was tested by real time PCR. Cell proliferation was evaluated by MTT assay. Cell cycle was determined by flow cytometry. Bcl-2, Bax, and survivin protein levels were detected by Western blot. MG3 expression significantly increased in Hep2 cells compared with control (P &lt; 0.05). Hep2 cell OD value and cell percentage in S phase were obviously declined, while cell apoptosis were markedly enhanced (P &lt; 0.05). OD value and cell percentage in S phase apparently reduced in 12 h, 24 h, and 48 h from experimental group (P &lt; 0.05). Bcl-2 and survivin protein downregulated, while Bax protein elevated in experimental group following time extension (P &lt; 0.05). MG3 overexpression inhibited laryngeal cancer Hep2 cell proliferation and arrested cell cycle with time dependence, which may achieve by suppressing Bcl-2 and survivin protein, and facilitating Bax protein expression.</description><identifier>EISSN: 1936-2625</identifier><identifier>PMID: 31966591</identifier><language>eng</language><publisher>United States: e-Century Publishing Corporation</publisher><subject>Original</subject><ispartof>International journal of clinical and experimental pathology, 2017-01, Vol.10 (7), p.7475-7480</ispartof><rights>IJCEP Copyright © 2017.</rights><rights>IJCEP Copyright © 2017 2017</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965295/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965295/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31966591$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Yuan</creatorcontrib><creatorcontrib>Zhou, Xuehua</creatorcontrib><creatorcontrib>Tao, Chenjuan</creatorcontrib><creatorcontrib>Chen, Chaohui</creatorcontrib><creatorcontrib>Cui, Caixia</creatorcontrib><creatorcontrib>Dai, Lili</creatorcontrib><creatorcontrib>Wu, Honglin</creatorcontrib><creatorcontrib>Wei, Qingyu</creatorcontrib><creatorcontrib>Luo, Song</creatorcontrib><title>The impact of lncRNA MG3 on laryngeal cancer cell growth, cycle, and apoptosis related factors</title><title>International journal of clinical and experimental pathology</title><addtitle>Int J Clin Exp Pathol</addtitle><description>Laryngeal cancer is a common head and neck malignant tumor. Long non-coding RNA (lncRNA) is a kind of RNA at the length more than 200 nucleotides that cannot code protein. They widely exist in the human genome. Maternally expressed gene 3 (MG3) is a kind of lncRNA that is associated with various malignant tumors development. This study explores the influence of MG3 on laryngeal cancer Hep-2 cell growth, cycle, and apoptosis related factors. Hep2 cells transfected by MG3 were treated as experimental group, while untransfected Hep2 cells were treated as control. MG3 expression was tested by real time PCR. Cell proliferation was evaluated by MTT assay. Cell cycle was determined by flow cytometry. Bcl-2, Bax, and survivin protein levels were detected by Western blot. MG3 expression significantly increased in Hep2 cells compared with control (P &lt; 0.05). Hep2 cell OD value and cell percentage in S phase were obviously declined, while cell apoptosis were markedly enhanced (P &lt; 0.05). OD value and cell percentage in S phase apparently reduced in 12 h, 24 h, and 48 h from experimental group (P &lt; 0.05). Bcl-2 and survivin protein downregulated, while Bax protein elevated in experimental group following time extension (P &lt; 0.05). MG3 overexpression inhibited laryngeal cancer Hep2 cell proliferation and arrested cell cycle with time dependence, which may achieve by suppressing Bcl-2 and survivin protein, and facilitating Bax protein expression.</description><subject>Original</subject><issn>1936-2625</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNpVkE9LAzEUxBdBbK1-BcnRQxfyZ5NtLkIpWoWqIPVqyGbftivZZE1Spd_eFavoaQ5v-M3MO8rGRDKRU0H5KDuN8RVjQWiBT7IRI1IILsk4e1lvAbVdr01CvkHWmaeHObpfMuQdsjrs3Qa0RUY7AwEZsBZtgv9I2ykye2NhirSrke59n3xsIwpgdYIaNQPQh3iWHTfaRjg_6CR7vrleL27z1ePybjFf5T0VIuV1OQhwM8OyEJg3TA7NDQhemUKUoCmrgBNGS0wMLWaSN0ZXvBJEEypLYGySXX1z-13VQW3ApaCt6kPbDRuU1636f3HtVm38uxJScCr5ALg8AIJ_20FMqmvj11ztwO-ioqxgHEshZ4P14m_Wb8jPU9knnftyYw</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Li, Yuan</creator><creator>Zhou, Xuehua</creator><creator>Tao, Chenjuan</creator><creator>Chen, Chaohui</creator><creator>Cui, Caixia</creator><creator>Dai, Lili</creator><creator>Wu, Honglin</creator><creator>Wei, Qingyu</creator><creator>Luo, Song</creator><general>e-Century Publishing Corporation</general><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170101</creationdate><title>The impact of lncRNA MG3 on laryngeal cancer cell growth, cycle, and apoptosis related factors</title><author>Li, Yuan ; Zhou, Xuehua ; Tao, Chenjuan ; Chen, Chaohui ; Cui, Caixia ; Dai, Lili ; Wu, Honglin ; Wei, Qingyu ; Luo, Song</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p266t-d7266e5c8094605f39936ce65bc467ea23be5132701c24895fcab5b61a1297e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Original</topic><toplevel>online_resources</toplevel><creatorcontrib>Li, Yuan</creatorcontrib><creatorcontrib>Zhou, Xuehua</creatorcontrib><creatorcontrib>Tao, Chenjuan</creatorcontrib><creatorcontrib>Chen, Chaohui</creatorcontrib><creatorcontrib>Cui, Caixia</creatorcontrib><creatorcontrib>Dai, Lili</creatorcontrib><creatorcontrib>Wu, Honglin</creatorcontrib><creatorcontrib>Wei, Qingyu</creatorcontrib><creatorcontrib>Luo, Song</creatorcontrib><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of clinical and experimental pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yuan</au><au>Zhou, Xuehua</au><au>Tao, Chenjuan</au><au>Chen, Chaohui</au><au>Cui, Caixia</au><au>Dai, Lili</au><au>Wu, Honglin</au><au>Wei, Qingyu</au><au>Luo, Song</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The impact of lncRNA MG3 on laryngeal cancer cell growth, cycle, and apoptosis related factors</atitle><jtitle>International journal of clinical and experimental pathology</jtitle><addtitle>Int J Clin Exp Pathol</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>10</volume><issue>7</issue><spage>7475</spage><epage>7480</epage><pages>7475-7480</pages><eissn>1936-2625</eissn><abstract>Laryngeal cancer is a common head and neck malignant tumor. Long non-coding RNA (lncRNA) is a kind of RNA at the length more than 200 nucleotides that cannot code protein. They widely exist in the human genome. Maternally expressed gene 3 (MG3) is a kind of lncRNA that is associated with various malignant tumors development. This study explores the influence of MG3 on laryngeal cancer Hep-2 cell growth, cycle, and apoptosis related factors. Hep2 cells transfected by MG3 were treated as experimental group, while untransfected Hep2 cells were treated as control. MG3 expression was tested by real time PCR. Cell proliferation was evaluated by MTT assay. Cell cycle was determined by flow cytometry. Bcl-2, Bax, and survivin protein levels were detected by Western blot. MG3 expression significantly increased in Hep2 cells compared with control (P &lt; 0.05). Hep2 cell OD value and cell percentage in S phase were obviously declined, while cell apoptosis were markedly enhanced (P &lt; 0.05). OD value and cell percentage in S phase apparently reduced in 12 h, 24 h, and 48 h from experimental group (P &lt; 0.05). Bcl-2 and survivin protein downregulated, while Bax protein elevated in experimental group following time extension (P &lt; 0.05). MG3 overexpression inhibited laryngeal cancer Hep2 cell proliferation and arrested cell cycle with time dependence, which may achieve by suppressing Bcl-2 and survivin protein, and facilitating Bax protein expression.</abstract><cop>United States</cop><pub>e-Century Publishing Corporation</pub><pmid>31966591</pmid><tpages>6</tpages></addata></record>
fulltext fulltext
identifier EISSN: 1936-2625
ispartof International journal of clinical and experimental pathology, 2017-01, Vol.10 (7), p.7475-7480
issn 1936-2625
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6965295
source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
subjects Original
title The impact of lncRNA MG3 on laryngeal cancer cell growth, cycle, and apoptosis related factors
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T08%3A20%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20impact%20of%20lncRNA%20MG3%20on%20laryngeal%20cancer%20cell%20growth,%20cycle,%20and%20apoptosis%20related%20factors&rft.jtitle=International%20journal%20of%20clinical%20and%20experimental%20pathology&rft.au=Li,%20Yuan&rft.date=2017-01-01&rft.volume=10&rft.issue=7&rft.spage=7475&rft.epage=7480&rft.pages=7475-7480&rft.eissn=1936-2625&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E2343509698%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2343509698&rft_id=info:pmid/31966591&rfr_iscdi=true