MiRNA-134 suppresses esophageal squamous cell carcinoma progression by targeting FOXM1

Previous studies showed that the dysregulation of miRNAs was closely associated with cancer progression. The aim of this study was to verify whether miR-134, miR-10a, miR-29c, miR-942, miR-93, and miR-218 could inhibit esophageal squamous cell carcinoma (ESCC) cell invasion and migration. ESCC tissue and normal esophageal tissue adjacent to carcinoma from patients (54 cases) undergoing surgery were collected. RT-PCR was used to test the expression of miR-134, miR-10a, miR-29c, miR-942, miR-93, and miR-218 in these tissues. In addition, western blot was applied to test the expression of MMP-2, MMP-9, COL1A1, COL1A5 and FOXM1. In the vitro experiment, EC9706 cells were transfected with miR-134 mimics, then wound healing was employed to test the migratory ability of EC9706 cells. Transwell chambers was used to test the invasion ability of cells. The expression of MMP-2, MMP-9, COL1A1, COL1A5, and FOXM1 waas detected by western blot. In order to confirm whether FOXM1-3'-UTR was the target gene of miR-134, we performed a luciferase assay. FOXM1 over-expression plasmid was transfected to further confirm miR-134 played its role by targeting FOXM1. Our results showed that the expression of miR-134 was decreased in the ESCC tissue compared with normal esophageal tissue, (P