The Health-Promoting Potential of Salix spp. Bark Polar Extracts: Key Insights on Phenolic Composition and In Vitro Bioactivity and Biocompatibility

spp. have been exploited for energy generation, along with folk medicine use of bark extracts for antipyretic and analgesic benefits. Bark phenolic components, rather than salicin, have demonstrated interesting bioactivities, which may ensure the sustainable bioprospection of bark. Therefore, this study highlights the detailed phenolic characterization, as well as the in vitro antioxidant, anti-hypertensive, growth inhibitory effects, and biocompatibility of Brot., L., and L. bark polar extracts. Fifteen phenolic compounds were characterized by ultra-high-performance liquid chromatography-ultraviolet detection-mass spectrometry analysis, from which two flavan-3-ols, an acetophenone, five flavanones, and a flavonol were detected, for the first time, as their bark components. bark extracts demonstrated strong free radical scavenging activity (5.58-23.62 µg mL IC range), effective inhibition on angiotensin-I converting enzyme (58-84%), and bactericidal action at 1250-2500 µg mL (6-8 log CFU mL reduction range). All tested bark extracts did not show cytotoxic potential against Caco-2 cells, as well as Brot. and L. extracts at 625 and 1250 µg mL against HaCaT and L929 cells. These valuable findings can pave innovative and safer food, nutraceutical, and/or cosmetic applications of bark phenolic-containing fractions.