Bone morphogenetic protein 2 is a depot-specific regulator of human adipogenesis

Background Bone morphogenetic proteins (BMPs) regulate adipogenesis but it is not clear whether they influence regional adipose tissue (AT) development in humans. Objective To characterise BMP2 expression, BMP2-SMAD1/5/8 signalling, and BMP2′s potential effect on proliferation and adipogenesis in hu...

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Veröffentlicht in:International Journal of Obesity 2019-12, Vol.43 (12), p.2458-2468
Hauptverfasser: Denton, Nathan F., Eghleilib, Mohamed, Al-Sharifi, Sama, Todorčević, Marijana, Neville, Matt J., Loh, Nellie, Drakesmith, Alexander, Karpe, Fredrik, Pinnick, Katherine E.
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Sprache:eng
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Zusammenfassung:Background Bone morphogenetic proteins (BMPs) regulate adipogenesis but it is not clear whether they influence regional adipose tissue (AT) development in humans. Objective To characterise BMP2 expression, BMP2-SMAD1/5/8 signalling, and BMP2′s potential effect on proliferation and adipogenesis in human subcutaneous abdominal and gluteal AT and its constituent preadipocytes. Methods BMP2 expression was measured in whole AT and immortalised preadipocytes via qPCR and Western blot; secreted/circulating BMP2 was measured by ELISA. The effect of BMP2 on preadipocyte proliferation was evaluated using a fluorescent assay. BMP2′s effect on adipogenesis in immortalised preadipocytes was determined via qPCR of adipogenic markers and cellular triacylglycerol (TAG) accumulation. BMP2-SMAD1/5/8 signalling was assessed in immortalised preadipocytes via Western blot and qPCR of ID1 expression. Results BMP2 was expressed and released by abdominal and gluteal AT and preadipocytes. Exogenous BMP2 dose dependently promoted adipogenesis in abdominal preadipocytes only; 50 ng/ml BMP2 increased PPARG2 expression (10-fold compared to vehicle, p  
ISSN:0307-0565
1476-5497
DOI:10.1038/s41366-019-0421-1