Growth phase proteomics of the heterotrophic marine bacterium Ruegeria pomeroyi

The heterotrophic marine bacterium, Ruegeria pomeroyi , was experimentally cultured under environmentally realistic carbon conditions and with a tracer-level addition of 13 C-labeled leucine to track bacterial protein biosynthesis through growth phases. A combination of methods allowed observation of real-time bacterial protein production to understand metabolic priorities through the different growth phases. Over 2000 proteins were identified in each experimental culture from exponential and stationary growth phases. Within two hours of the 13 C-labeled leucine addition, R . pomeroyi significantly assimilated the newly encountered substrate into new proteins. This dataset provides a fundamental baseline for understanding growth phase differences in molecular physiology of a cosmopolitan marine bacterium. Measurement(s) proteomic profiling Technology Type(s) liquid chromatography-tandem mass spectrometry Factor Type(s) sample interval • biological replicate Sample Characteristic - Organism Ruegeria pomeroyi Sample Characteristic - Environment marine biome Machine-accessible metadata file describing the reported data: https://doi.org/10.6084/m9.figshare.10282922