Chronic adolescent stress sex-specifically alters central and peripheral neuro-immune reactivity in rats

•Chronic adolescent stress in rats primes neuroimmune reactivity in adulthood.•Adolescent stress potentiated NFκB-related genes post-LPS in the adult hippocampus.•Hippocampal IL1B post-LPS was enhanced by adolescent stress in adult females only.•Adolescent stress enhanced peripheral immune outcomes...

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Veröffentlicht in:Brain, behavior, and immunity behavior, and immunity, 2019-02, Vol.76, p.248-257
Hauptverfasser: Bekhbat, Mandakh, Howell, Paul A., Rowson, Sydney A., Kelly, Sean D., Tansey, Malú G., Neigh, Gretchen N.
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Sprache:eng
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Zusammenfassung:•Chronic adolescent stress in rats primes neuroimmune reactivity in adulthood.•Adolescent stress potentiated NFκB-related genes post-LPS in the adult hippocampus.•Hippocampal IL1B post-LPS was enhanced by adolescent stress in adult females only.•Adolescent stress enhanced peripheral immune outcomes post-LPS in adult males only.•Early life stress exerts differential long-term immune impact in males and females. Adversity during development is a reliable predictor of psychiatric disorders such as depression and anxiety which are increasingly recognized to have an immune component. We have previously demonstrated that chronic adolescent stress (CAS) in rats leads to depressive-like behavior in adulthood along with long-lasting changes to the hypothalamic-pituitary-adrenal axis and pro-inflammatory cytokine induction in the hippocampus. However, the mechanisms by which CAS promotes hippocampal inflammation are not yet defined. Here we tested the hypothesis that a history of CAS exaggerates induction of the pro-inflammatory NFκB pathway in the adult rat hippocampus without compromising the peripheral immune response. We also assessed potential sex differences because it is unclear whether females, who are twice as likely to suffer from mood disorders as males, are disproportionally affected by stress-primed inflammation. Male and female adolescent rats underwent a CAS paradigm or received no stress. Six weeks following the last stressor, all rats received a single systemic injection of either lipopolysaccharide or vehicle to unmask possible immune-priming effects of CAS. An NFκB signaling PCR array demonstrated that CAS exaggerated the expression of NFκB-related genes in the hippocampus of both males and females. Interestingly, targeted qPCR demonstrated that CAS potentiated the induction of hippocampal IL1B and REL mRNA in female rats only, suggesting that some immune effects of CAS are indeed sex-specific. In contrast to the hippocampal findings, indices of peripheral inflammation such as NFκB activity in the spleen, plasma IL-1β, IL-6, TNF-α, and corticosterone were not impacted by CAS in female rats. Despite showing no pro-inflammatory changes to hippocampal mRNA, male CAS rats displayed lower plasma corticosterone response to LPS at 2 h after injection followed by an exaggerated plasma IL-1β response at 4 h. This potentially blunted corticosterone response coupled with excessive innate immune signaling in the periphery is consistent with possible glucocort
ISSN:0889-1591
1090-2139
DOI:10.1016/j.bbi.2018.12.005