Analysis of tRNACys processing in the absence of CCAase in Bacillus subtilis

In Bacillus subtilis , the tRNA Cys lacks an encoded CCA 3′ end. To gain insight into the role of CCAase and RNases in tRNA Cys processing, several mutant strains were generated. Northern blot and RT-PCR results suggest that enzymes other than CCAase can participate in CCA addition at the 3′ end of...

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Veröffentlicht in:	Brazilian journal of microbiology 2019-07, Vol.50 (3), p.613-618
Hauptverfasser:	Campos Guillén, Juan, Arvizu Gómez, Jackeline Lizzeta, Jones, George H., Hernández Flores, José Luis, Ramos López, Miguel Angel, Cruz Hernández, Andrés, Romero Gómez, Sergio
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus subtilis Bacterial Bacterial, Fungal and Virus Molecular Biology - Short Communication Biomedical and Life Sciences Food Microbiology Fungal and Virus Molecular Biology - Short Communication Life Sciences Medical Microbiology Microbial Ecology Microbial Genetics and Genomics Microbiology Mycology Polymerase chain reaction
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Zusammenfassung:	In Bacillus subtilis , the tRNA Cys lacks an encoded CCA 3′ end. To gain insight into the role of CCAase and RNases in tRNA Cys processing, several mutant strains were generated. Northern blot and RT-PCR results suggest that enzymes other than CCAase can participate in CCA addition at the 3′ end of the immature tRNA Cys .
ISSN:	1517-8382 1678-4405
DOI:	10.1007/s42770-019-00075-5