CidR and CcpA Synergistically Regulate Staphylococcus aureus cidABC Expression
The death and lysis of a subpopulation of cells during biofilm development benefit the whole bacterial population through the release of an important component of the biofilm matrix, extracellular DNA. Previously, we have demonstrated that these processes are affected by the gene products of the ope...
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Veröffentlicht in: | Journal of bacteriology 2019-12, Vol.201 (23), p.1 |
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Sprache: | eng |
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Zusammenfassung: | The death and lysis of a subpopulation of
cells during biofilm development benefit the whole bacterial population through the release of an important component of the biofilm matrix, extracellular DNA. Previously, we have demonstrated that these processes are affected by the gene products of the
operon, the expression of which is controlled by the LysR-type transcriptional regulator, CidR. In this study, we characterized
- and
-acting elements essential for the induction of the
operon. In addition to a CidR-binding site located within the
promoter region, sequence analysis revealed the presence of a putative catabolite responsive element (
box), suggestive of the involvement of the catabolite control protein A (CcpA) in the regulation of
expression. This was confirmed using electrophoretic mobility shift assays and real-time reverse transcriptase PCR analysis demonstrating the direct positive control of
transcription by the master regulator of carbon metabolism. Furthermore, the importance of CcpA and the identified
site for the induction of the
operon was demonstrated by examining the expression of P
reporter fusions in various mutant strains in which the genes involved in carbon metabolism and carbon catabolite repression were disrupted. Together the results of this study demonstrate the necessity of both transcriptional regulators, CidR and CcpA, for the induction of the
operon and reveal the complexity of molecular interactions controlling its expression.
This work focuses on the characterization of
- and
-acting elements essential for the induction of the
operon in
The results of this study are the first to demonstrate the synergistic control of
expression by transcriptional regulators CidR and CcpA during carbohydrate metabolism. We established that the full induction of
expression depends on the metabolic state of bacteria and requires both CidR and CcpA. Together, these findings delineate regulatory control of
expression under different metabolic conditions and provide important new insights into our understanding of cell death mechanisms during biofilm development in
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ISSN: | 0021-9193 1098-5530 |
DOI: | 10.1128/JB.00371-19 |