Rapid and Definitive Analysis of In Vitro DNA Methylation by Nano-electrospray Ionization Mass Spectrometry

CpG methylation of DNA is an epigenetic marker that is highly related to the regulation of transcription initiation. For analysis of CpG methylation in genomic DNA sequences, bisulfite-induced modification in combination with polymerase chain reaction (PCR) is usually utilized, but it cannot be stra...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of the American Society for Mass Spectrometry 2019-11, Vol.30 (11), p.2335-2346
Hauptverfasser:	Ushijima, Hiroshi, Maekawa, Rena, Igarashi, Eri, Akashi, Satoko
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Bioinformatics Biology Biotechnology Chemistry Chemistry and Materials Science CpG Islands - genetics Deoxyribonucleic acid Digestion DNA DNA - genetics DNA methylation DNA Methylation - genetics Electrospraying Escherichia coli Fragments Gene expression Gene sequencing Ionization Ions Levels Liquid chromatography Mass spectrometry Nanotechnology - methods Nuclease Oligomers Organic Chemistry Polymerase chain reaction Proteomics Research Article Scientific imaging Spectrometry, Mass, Electrospray Ionization - methods Spectroscopy
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	CpG methylation of DNA is an epigenetic marker that is highly related to the regulation of transcription initiation. For analysis of CpG methylation in genomic DNA sequences, bisulfite-induced modification in combination with polymerase chain reaction (PCR) is usually utilized, but it cannot be straightforwardly applied to methylated short- and middle-sized DNAs, such as
ISSN:	1044-0305 1879-1123
DOI:	10.1007/s13361-019-02304-5