Macroscopic and Microscopic Properties of a Cloned Glutamate Transporter/Chloride Channel
The behavior of a Cl- channel associated with a glutamate transporter was studied using intracellular and patch recording techniques in Xenopus oocytes injected with human EAAT1 cRNA. Channels could be activated by application of glutamate to either face of excised membrane patches. The channel exhi...
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| Veröffentlicht in: | The Journal of neuroscience 1998-10, Vol.18 (19), p.7650-7661 |
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| container_title | The Journal of neuroscience |
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| creator | Wadiche, Jacques I Kavanaugh, Michael P |
| description | The behavior of a Cl- channel associated with a glutamate transporter was studied using intracellular and patch recording techniques in Xenopus oocytes injected with human EAAT1 cRNA. Channels could be activated by application of glutamate to either face of excised membrane patches. The channel exhibited strong selectivity for amphipathic anions and had a minimum pore diameter of approximately 5A. Glutamate flux exhibited a much greater temperature dependence than Cl- flux. Stationary and nonstationary noise analysis was consistent with a sub-femtosiemen Cl- conductance and a maximum channel Po |
| doi_str_mv | 10.1523/jneurosci.18-19-07650.1998 |
| format | Article |
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Channels could be activated by application of glutamate to either face of excised membrane patches. The channel exhibited strong selectivity for amphipathic anions and had a minimum pore diameter of approximately 5A. Glutamate flux exhibited a much greater temperature dependence than Cl- flux. Stationary and nonstationary noise analysis was consistent with a sub-femtosiemen Cl- conductance and a maximum channel Po << 1. The glutamate binding rate was similar to estimates for receptor binding. After glutamate binding, channels activated rapidly followed by a relaxation phase. 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Channels could be activated by application of glutamate to either face of excised membrane patches. The channel exhibited strong selectivity for amphipathic anions and had a minimum pore diameter of approximately 5A. Glutamate flux exhibited a much greater temperature dependence than Cl- flux. Stationary and nonstationary noise analysis was consistent with a sub-femtosiemen Cl- conductance and a maximum channel Po << 1. The glutamate binding rate was similar to estimates for receptor binding. After glutamate binding, channels activated rapidly followed by a relaxation phase. Differences in the macroscopic kinetics of channels activated by concentration jumps of L-glutamate or D-aspartate were correlated with differences in uptake kinetics, indicating a close correspondence of channel gating to state transitions in the transporter cycle.</description><subject>Amino Acid Transport System X-AG</subject><subject>Animals</subject><subject>Anions - pharmacokinetics</subject><subject>Aspartic Acid - pharmacology</subject><subject>ATP-Binding Cassette Transporters - genetics</subject><subject>ATP-Binding Cassette Transporters - metabolism</subject><subject>Biological Transport - genetics</subject><subject>Brain Chemistry - physiology</subject><subject>Chloride Channels - genetics</subject><subject>Chloride Channels - metabolism</subject><subject>Cloning, Molecular</subject><subject>Electric Conductivity</subject><subject>Glutamic Acid - pharmacokinetics</subject><subject>Humans</subject><subject>Ion Channel Gating - physiology</subject><subject>Kinetics</subject><subject>Membrane Potentials - physiology</subject><subject>Oocytes - physiology</subject><subject>Patch-Clamp Techniques</subject><subject>Potassium - pharmacokinetics</subject><subject>Protons</subject><subject>Sodium - pharmacokinetics</subject><subject>Xenopus</subject><issn>0270-6474</issn><issn>1529-2401</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUFv1DAQha0KVJbSn1Ap4gCntHbs2DEHpCpaSlFLEbSHniyvM2lcOXZqJ6z49yTsqsBpNHpvvhnNQ-gtwaekLOjZo4cphmTsKalyInMseDlLUlYHaDU7ZF4wTF6gFS4EzjkT7BV6ndIjxlhgIg7RoRSsIJSv0P21NgsqDNZk2jfZtf3bf4thgDhaSFloM53VLnhosgs3jbrXI2S3Ufs0hDhCPKs7F6JtIKs77T24N-hlq12C4309Qnef1rf15_zq5uKyPr_KTSn4mPMNkVAY0FqWlFWGmA0Q02JOuWzKFksQwBgrtOCMl6wxLa-AElxUtDSMc3qEPu64w7TpoTHgx6idGqLtdfylgrbqf8XbTj2En4oLSTFeAO_2gBieJkij6m0y4Jz2EKakBJWUU7oYP-yMy4dShPZ5CcFqCUZ9-bq--37zo75UpFJEqj_BqCWYefjk3zOfR_dJzPr7nd7Zh25rI6jUa-dmN1Hb7XbHW3D0N2vFm5U</recordid><startdate>19981001</startdate><enddate>19981001</enddate><creator>Wadiche, Jacques I</creator><creator>Kavanaugh, Michael P</creator><general>Soc Neuroscience</general><general>Society for Neuroscience</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19981001</creationdate><title>Macroscopic and Microscopic Properties of a Cloned Glutamate Transporter/Chloride Channel</title><author>Wadiche, Jacques I ; Kavanaugh, Michael P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c576t-6b19e2ceaa95348c1cbe1cf06369d5f09e7e4442a764654dcf68e3102835c4663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Transport System X-AG</topic><topic>Animals</topic><topic>Anions - pharmacokinetics</topic><topic>Aspartic Acid - pharmacology</topic><topic>ATP-Binding Cassette Transporters - genetics</topic><topic>ATP-Binding Cassette Transporters - metabolism</topic><topic>Biological Transport - genetics</topic><topic>Brain Chemistry - physiology</topic><topic>Chloride Channels - genetics</topic><topic>Chloride Channels - metabolism</topic><topic>Cloning, Molecular</topic><topic>Electric Conductivity</topic><topic>Glutamic Acid - pharmacokinetics</topic><topic>Humans</topic><topic>Ion Channel Gating - physiology</topic><topic>Kinetics</topic><topic>Membrane Potentials - physiology</topic><topic>Oocytes - physiology</topic><topic>Patch-Clamp Techniques</topic><topic>Potassium - pharmacokinetics</topic><topic>Protons</topic><topic>Sodium - pharmacokinetics</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wadiche, Jacques I</creatorcontrib><creatorcontrib>Kavanaugh, Michael P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wadiche, Jacques I</au><au>Kavanaugh, Michael P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Macroscopic and Microscopic Properties of a Cloned Glutamate Transporter/Chloride Channel</atitle><jtitle>The Journal of neuroscience</jtitle><addtitle>J Neurosci</addtitle><date>1998-10-01</date><risdate>1998</risdate><volume>18</volume><issue>19</issue><spage>7650</spage><epage>7661</epage><pages>7650-7661</pages><issn>0270-6474</issn><eissn>1529-2401</eissn><abstract>The behavior of a Cl- channel associated with a glutamate transporter was studied using intracellular and patch recording techniques in Xenopus oocytes injected with human EAAT1 cRNA. 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Differences in the macroscopic kinetics of channels activated by concentration jumps of L-glutamate or D-aspartate were correlated with differences in uptake kinetics, indicating a close correspondence of channel gating to state transitions in the transporter cycle.</abstract><cop>United States</cop><pub>Soc Neuroscience</pub><pmid>9742136</pmid><doi>10.1523/jneurosci.18-19-07650.1998</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of neuroscience, 1998-10, Vol.18 (19), p.7650-7661 |
| issn | 0270-6474 1529-2401 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Amino Acid Transport System X-AG Animals Anions - pharmacokinetics Aspartic Acid - pharmacology ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - metabolism Biological Transport - genetics Brain Chemistry - physiology Chloride Channels - genetics Chloride Channels - metabolism Cloning, Molecular Electric Conductivity Glutamic Acid - pharmacokinetics Humans Ion Channel Gating - physiology Kinetics Membrane Potentials - physiology Oocytes - physiology Patch-Clamp Techniques Potassium - pharmacokinetics Protons Sodium - pharmacokinetics Xenopus |
| title | Macroscopic and Microscopic Properties of a Cloned Glutamate Transporter/Chloride Channel |
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