Macroscopic and Microscopic Properties of a Cloned Glutamate Transporter/Chloride Channel

The behavior of a Cl- channel associated with a glutamate transporter was studied using intracellular and patch recording techniques in Xenopus oocytes injected with human EAAT1 cRNA. Channels could be activated by application of glutamate to either face of excised membrane patches. The channel exhi...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	The Journal of neuroscience 1998-10, Vol.18 (19), p.7650-7661
Hauptverfasser:	Wadiche, Jacques I, Kavanaugh, Michael P
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Transport System X-AG Animals Anions - pharmacokinetics Aspartic Acid - pharmacology ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - metabolism Biological Transport - genetics Brain Chemistry - physiology Chloride Channels - genetics Chloride Channels - metabolism Cloning, Molecular Electric Conductivity Glutamic Acid - pharmacokinetics Humans Ion Channel Gating - physiology Kinetics Membrane Potentials - physiology Oocytes - physiology Patch-Clamp Techniques Potassium - pharmacokinetics Protons Sodium - pharmacokinetics Xenopus
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The behavior of a Cl- channel associated with a glutamate transporter was studied using intracellular and patch recording techniques in Xenopus oocytes injected with human EAAT1 cRNA. Channels could be activated by application of glutamate to either face of excised membrane patches. The channel exhibited strong selectivity for amphipathic anions and had a minimum pore diameter of approximately 5A. Glutamate flux exhibited a much greater temperature dependence than Cl- flux. Stationary and nonstationary noise analysis was consistent with a sub-femtosiemen Cl- conductance and a maximum channel Po
ISSN:	0270-6474 1529-2401
DOI:	10.1523/jneurosci.18-19-07650.1998