Genetically Programmable Self‐Regenerating Bacterial Hydrogels

A notable challenge for the design of engineered living materials (ELMs) is programming a cellular system to assimilate resources from its surroundings and convert them into macroscopic materials with specific functions. Here, an ELM that uses Escherichia coli as its cellular chassis and engineered curli nanofibers as its extracellular matrix component is demonstrated. Cell‐laden hydrogels are created by concentrating curli‐producing cultures. The rheological properties of the living hydrogels are modulated by genetically encoded factors and processing steps. The hydrogels have the ability to grow and self‐renew when placed under conditions that facilitate cell growth. Genetic programming enables the gels to be customized to interact with different tissues of the gastrointestinal tract selectively. This work lays a foundation for the application of ELMs with therapeutic functions and extended residence times in the gut. Biofabricated hydrogels have the ability to grow and self‐regenerate in the gastrointestinal tract. Genetic programming enables customization to interact with different tissues of the gastrointestinal tract selectively and to fabricate living hydrogel materials. This novel engineered living material lays a foundation for therapeutic applications in the gut, especially those that may benefit from a cohesive material with extended residence times.