Fast isolation of RNA to detect expression of tumor markers

Fast isolation of RNA to detect expression of tumor markers

The expression status of several tumor‐related proteins is of great interest in clinical examination and research. As a completion to conventional antibody staining, RT‐PCR is often used today. Reliable isolation of RNA from a low number of cells is very often a critical stage of such an examination...

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Veröffentlicht in:Journal of clinical laboratory analysis 1997, Vol.11 (6), p.340-342
Hauptverfasser: Schenk, Jörg A., Hillebrand, Timo, Lübbe, Lieselotte, Heymann, Stephan, Böttger, Michael, Micheel, Burkhard, Bendzko, Peter
Format: Artikel
Sprache:eng
Schlagworte:
ATP-Binding Cassette, Sub-Family B, Member 1 - analysis
ATP-Binding Cassette, Sub-Family B, Member 1 - genetics
Biomarkers, Tumor - analysis
breast cancer
c-erb-b2
carcinoembryonic antigen
Carcinoembryonic Antigen - analysis
Carcinoembryonic Antigen - genetics
carrier method
Gene Expression
Humans
MaTu
mdr1
Original
PCR
Receptor, ErbB-2 - analysis
Receptor, ErbB-2 - genetics
RNA, Neoplasm - isolation & purification
RNA-Directed DNA Polymerase
Tumor Cells, Cultured
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Beschreibung
Zusammenfassung:The expression status of several tumor‐related proteins is of great interest in clinical examination and research. As a completion to conventional antibody staining, RT‐PCR is often used today. Reliable isolation of RNA from a low number of cells is very often a critical stage of such an examination. We demonstrate here a simple and fast method to isolate RNA from only 10,000 cells and applied it to the detection of CEA, c‐ERB‐B2, and mdr‐1 as often studied models for tumor markers. J. Clin. Lab. Anal. 11:340–342, 1997. © 1997 Wiley‐Liss, Inc.
ISSN:0887-8013
1098-2825
DOI:10.1002/(SICI)1098-2825(1997)11:6<340::AID-JCLA5>3.0.CO;2-9