A novel magnet-based scratch method for standardisation of wound-healing assays

A novel magnet-based scratch method for standardisation of wound-healing assays

A novel magnetic scratch method achieves repeatability, reproducibility and geometric control greater than pipette scratch assays and closely approximating the precision of cell exclusion assays while inducing the cell injury inherently necessary for wound healing assays. The magnetic scratch is aff...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Scientific reports 2019-09, Vol.9 (1), p.12625-9, Article 12625
Hauptverfasser: Fenu, M., Bettermann, T., Vogl, C., Darwish-Miranda, N., Schramel, J., Jenner, F., Ribitsch, I.
Format: Artikel
Sprache:eng
Schlagworte:
631/1647/767
631/80/84/2334
Animals
Biological Assay - standards
Cell adhesion & migration
Cell culture
Cell injury
Cells, Cultured
Chondrocytes - cytology
Horses
Humanities and Social Sciences
Laboratories
Magnetic Phenomena
Methods
multidisciplinary
Reference Standards
Reproducibility
Science
Science (multidisciplinary)
Standard deviation
Veterinary medicine
Wound Healing
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A novel magnetic scratch method achieves repeatability, reproducibility and geometric control greater than pipette scratch assays and closely approximating the precision of cell exclusion assays while inducing the cell injury inherently necessary for wound healing assays. The magnetic scratch is affordable, easily implemented and standardisable and thus may contribute toward better comparability of data generated in different studies and laboratories.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-48930-7