A flow-through microfluidic system for the detection of circulating melanoma cells

We report the fabrication of polyaniline nanofiber (PANI)-modified screen-printed electrode (PANI/SPE) incorporated in a poly-dimethylsiloxane (PDMS) microfluidic channel for the detection of circulating tumor cells. We employed this device to detect melanoma skin cancer cells through specific immunogenic binding of cell surface biomarker melanocortin 1 receptor (MC1R) to anti-MC1R antibody. The antibody-functionalized PANI/SPE was used in batch-continuous flow-through fashion. An aqueous cell suspension of ferri/ferrocyanide at a flow rate of 1.5 mL/min was passed over the immunosensor, which allowed for continuous electrochemical measurements. The sensor performed exceptionally well affording an ultralow limit of quantification of 1 melanoma cell/mL, both in buffer and when mixed with peripheral blood mononuclear cells, and the response was log-linear over the range of 10–9000 melanoma cells/10 mL. •A microfluidics-based immunoelectrochemical sensor was developed.•An ultralow limit of quantification of 1 cell/mL was achieved.•The sensor performance was verified via cell culture experiments.•The sensor response is stable, repeatable, and linear over a wide range.•This system is potentially useful for rapid detection of circulating tumor cells.