3- O -Methyl-Alkylgallates Inhibit Fatty Acid Desaturation in Mycobacterium tuberculosis

In the quest for new antibacterial lead structures, activity screening against identified antitubercular effects of gallic acid derivatives isolated from the Nigerian mistletoe Structure-activity relationship studies indicated that 3- -methyl-alkylgallates comprising aliphatic ester chains with four to eight carbon atoms showed the strongest growth inhibition against , with a MIC of 6.25 μM. Furthermore, the most active compounds (3- -methyl-butyl-, 3- -methyl-hexylgallate, and 3- -methyl-octylgallate) were devoid of cytotoxicity against various human cell lines. Furthermore, 3- -methyl-butylgallate showed favorable absorption, distribution, metabolism, and excretion (ADME) criteria, with a of 6.2 × 10 cm/s, and it did not inhibit P-glycoprotein (P-gp), CYP1A2, CYP2B6 or CYP3A4. Whole-genome sequencing of spontaneous resistant mutants indicated that the compounds target the stearoyl-coenzyme A (stearoyl-CoA) delta-9 desaturase DesA3 and thereby inhibit oleic acid synthesis. Supplementation assays demonstrated that oleic acid addition to the culture medium antagonizes the inhibitory properties of gallic acid derivatives and that sodium salts of saturated palmitic and stearic acid did not show compensatory effects. The moderate bactericidal effect of 3- -methyl-butylgallate in monotreatment was synergistically enhanced in combination treatment with isoniazid, leading to sterilization in liquid culture.