High frequency of the exoU+/exoS+ genotype associated with multidrug-resistant “high-risk clones” of Pseudomonas aeruginosa clinical isolates from Peruvian hospitals
The type III secretion system of Pseudomonas aeruginosa is an important virulence factor contributing to the cytotoxicity and the invasion process of this microorganism. The current study aimed to determine the presence of the exoU +/ exoS + genotype in P . aeruginosa clinical isolates. The presence...
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Veröffentlicht in: | Scientific reports 2019-07, Vol.9 (1), p.10874-13, Article 10874 |
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Zusammenfassung: | The type III secretion system of
Pseudomonas aeruginosa
is an important virulence factor contributing to the cytotoxicity and the invasion process of this microorganism. The current study aimed to determine the presence of the
exoU
+/
exoS
+ genotype in
P
.
aeruginosa
clinical isolates. The presence of
exoS
,
exoT
,
exoU
and
exoY
was determined in 189
P
.
aeruginosa
by PCR, and the presence/absence of
exoU
was analysed according to source infection, clonal relationships, biofilm formation, motility and antimicrobial susceptibility. The
gyrA
,
parC
,
oprD
, efflux pump regulators and β-lactamases genes were also analysed by PCR/sequencing. The
exoS
,
exoT
and
exoY
genes were found in 100% of the isolates. Meanwhile,
exoU
was present in 43/189 (22.8%) of the isolates, being significantly associated with multidrug resistance, extensively drug resistance as well as with higher level quinolone resistance. However, the presence of β-lactamases, mutations in
gyrA
and
parC
, and relevant modifications in efflux pumps and OprD were not significantly associated with
exoU
+ isolates. MLST analysis of a subset of 25 isolates showed 8 different STs displaying the
exoU
+/
exoS
+ genotype. The MDR basis of the
exoU
+ isolates remain to be elucidated. Furthermore, the clinical implications and spread of
exoU
+/
exoS
+
P
.
aeruginosa
isolates need to be established. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-019-47303-4 |