Proof of concept for quantitative urine NMR metabolomics pipeline for large-scale epidemiology and genetics

Abstract Background Quantitative molecular data from urine are rare in epidemiology and genetics. NMR spectroscopy could provide these data in high throughput, and it has already been applied in epidemiological settings to analyse urine samples. However, quantitative protocols for large-scale applic...

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Veröffentlicht in:International journal of epidemiology 2019-06, Vol.48 (3), p.978-993
Hauptverfasser: Tynkkynen, Tuulia, Wang, Qin, Ekholm, Jussi, Anufrieva, Olga, Ohukainen, Pauli, Vepsäläinen, Jouko, Männikkö, Minna, Keinänen-Kiukaanniemi, Sirkka, Holmes, Michael V, Goodwin, Matthew, Ring, Susan, Chambers, John C, Kooner, Jaspal, Järvelin, Marjo-Riitta, Kettunen, Johannes, Hill, Michael, Davey Smith, George, Ala-Korpela, Mika
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Sprache:eng
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Zusammenfassung:Abstract Background Quantitative molecular data from urine are rare in epidemiology and genetics. NMR spectroscopy could provide these data in high throughput, and it has already been applied in epidemiological settings to analyse urine samples. However, quantitative protocols for large-scale applications are not available. Methods We describe in detail how to prepare urine samples and perform NMR experiments to obtain quantitative metabolic information. Semi-automated quantitative line shape fitting analyses were set up for 43 metabolites and applied to data from various analytical test samples and from 1004 individuals from a population-based epidemiological cohort. Novel analyses on how urine metabolites associate with quantitative serum NMR metabolomics data (61 metabolic measures; n = 995) were performed. In addition, confirmatory genome-wide analyses of urine metabolites were conducted (n = 578). The fully automated quantitative regression-based spectral analysis is demonstrated for creatinine and glucose (n = 4548). Results Intra-assay metabolite variations were mostly
ISSN:0300-5771
1464-3685
DOI:10.1093/ije/dyy287