One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus

One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus

To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 102.1 TCID50 for CDV, 101.9 TCID50 for CPV and 103 copies for CaKoV. This method did not amplify nonsp...

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Veröffentlicht in:Journal of Veterinary Medical Science 2019, Vol.81(7), pp.1040-1042
Hauptverfasser: LIU, Dafei, LIU, Fei, GUO, Dongchun, HU, Xiaoliang, LI, Zhijie, LI, Zhigang, MA, Jianzhang, LIU, Chunguo
Format: Artikel
Sprache:eng
Schlagworte:
Canine distemper
canine distemper virus (CDV)
canine kobuvirus (CaKoV)
canine parvovirus (CPV)
Deoxyribonucleic acid
Detection limits
DNA
Epidemiology
one-step triplex PCR/RT-PCR
Parvoviruses
Polymerase chain reaction
Ribonucleic acid
RNA
RNA viruses
Virology
Viruses
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Beschreibung
Zusammenfassung:To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 102.1 TCID50 for CDV, 101.9 TCID50 for CPV and 103 copies for CaKoV. This method did not amplify nonspecific DNA or RNA from other canine viruses. Therefore, the assay provides a sensitive tool for the rapid clinical detection and epidemiological surveillance of CDV, CPV and CaKoV in dogs.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.17-0442